TY - JOUR
T1 - A simple and quantitative method for analysis of methoxyamine by capillary zone electrophoresis
AU - Liao, Yu Chieh
AU - Syu, Mei Jywan
AU - Xu, Yan
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005
Y1 - 2005
N2 - Methoxyamine (MX) is a potential new chemotherapeutic agent. In structure, MX lacks a chromophore for spectrophotometric detection. Derivatization of MX with chromophoric 4-(diethylamino)benzaldehyde (DEAB) was performed and the resultant MX derivative (DBMOH+) can be analyzed by capillary zone electrophoresis (CZE) with ultraviolet (UV) detection at 200 nm. The limit of detection (SD/S = 3.3) of the method for MX was 2.50 μM with a 2 s injection at the pressure of 3.45 kPa. The linear calibration range for MX was 5.00-500 μM using N,N-dimethyl-p-toluidine (DMPT) as the internal standard. The intra- and inter-assay precision and accuracy of the method were ≤4% and within 98.4-104%, respectively. This method may be used for the quantitative determination of MX in pharmaceutical preparations.
AB - Methoxyamine (MX) is a potential new chemotherapeutic agent. In structure, MX lacks a chromophore for spectrophotometric detection. Derivatization of MX with chromophoric 4-(diethylamino)benzaldehyde (DEAB) was performed and the resultant MX derivative (DBMOH+) can be analyzed by capillary zone electrophoresis (CZE) with ultraviolet (UV) detection at 200 nm. The limit of detection (SD/S = 3.3) of the method for MX was 2.50 μM with a 2 s injection at the pressure of 3.45 kPa. The linear calibration range for MX was 5.00-500 μM using N,N-dimethyl-p-toluidine (DMPT) as the internal standard. The intra- and inter-assay precision and accuracy of the method were ≤4% and within 98.4-104%, respectively. This method may be used for the quantitative determination of MX in pharmaceutical preparations.
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U2 - 10.1080/10826070500187665
DO - 10.1080/10826070500187665
M3 - Article
AN - SCOPUS:25444493867
SN - 1082-6076
VL - 28
SP - 2433
EP - 2443
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 15
ER -