A simplified gene-specific screen for Y chromosome deletions in infertile men

Yen Ni Teng, Ying Hung Lin, Yung Chieh Tsai, Chao Chin Hsu, Pao-Lin Kuo, YungMing Lin

研究成果: Article同行評審

10 引文 斯高帕斯(Scopus)


Objective: To test the diagnostic efficiency of a gene-specific, five-marker screening strategy for the detection of Y chromosome deletions. Design: Prospective case study. Setting: University genetics laboratory and reproductive clinics. Patient(s): Six hundred twenty-seven infertile men and 212 fertile men. Intervention(s): Peripheral blood samples were screened for Y chromosome deletions in a triple-blind fashion using three protocols: protocol I consisted of five gene-specific markers, including USP9Y, DBY, SMCY, RBM1, and DAZ; protocol II included 14 gene-specific markers; and protocol III consisted of six sequence-tagged sites (STSs) markers recommended by EAA/EMQN. Main Outcome Measure(s): Deletion status of Y chromosome genes or sequence-tagged sites. Result(s): Protocols I and II identified the same 41 infertile patients with Y deletions. Protocol III identified 38 infertile patients with Y deletions, and all 38 patients were also identified by protocols I and II. One patient with isolated USP9Y deletion and two patients with isolated DBY deletions, as detected by protocols I and II, could not be identified by protocol III. Conclusion(s): We observed mostly consistent results between our protocols and the EAA/EMQN protocol. This gene-specific, five-marker screening panel provides the same diagnostic efficiency as the EAA/EMQN protocol and may be considered an alternative to the EAA/EMQN protocol.

頁(從 - 到)1291-1300
期刊Fertility and Sterility
出版狀態Published - 2007 六月 1

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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