A single nucleotide in stem loop II of 5′-untranslated region contributes to virulence of Enterovirus 71 in mice

Ming Te Yeh, Shainn Wei Wang, Chun Keung Yu, Kuei Hsiang Lin, Huan Yao Lei, Ih Jen Su, Jen Ren Wang

研究成果: Article

44 引文 斯高帕斯(Scopus)

摘要

Background: Enterovirus 71 (EV71) has emerged as a neuroinvasive virus responsible for several large outbreaks in the Asia-Pacific region while virulence determinant remains unexplored. Principal Findings: In this report, we investigated increased virulence of unadapted EV71 clinical isolate 237 as compared with isolate 4643 in mice. A fragment 12 nucleotides in length in stem loop (SL) II of 237 5′-untranslated region (UTR) visibly reduced survival time and rate in mice was identified by constructing a series of infectious clones harboring chimeric 5′-UTR. In cells transfected with bicistronic plasmids, and replicon RNAs, the 12-nt fragment of isolate 237 enhanced translational activities and accelerated replication of subgenomic EV71. Finally, single nucleotide change from cytosine to uridine at base 158 in this short fragment of 5′-UTR was proven to reduce viral translation and EV71 virulence in mice. Results collectively indicated a pivotal role of novel virulence determinant C158 on virus translation in vitro and EV71 virulence in vivo. Conclusions: These results presented the first reported virulence determinant in EV71 5′-UTR and first position discovered from unadapted isolates.

原文English
文章編號e27082
期刊PloS one
6
發行號10
DOIs
出版狀態Published - 2011 十一月 1

    指紋

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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