A study on enhanced fluorescence signal-to-noise by using the stray light shutter for quantitative PCR chip

Liang Chieh Chao, Chun Han Chou, Hsin Yi Tsai, Kuo Cheng Huang, Dar Bin Shieh

研究成果: Conference contribution

摘要

Quantitative polymerase chain reaction (qPCR) is the most important inspection technique for virus, especially for coronavirus disease, in this year. The qPCR chip and device were planned to develop because of the characteristics of fast inspection time, high accuracy and small system volume. Therein, the fluorescence intensity was the important signal in qPCR device, which represented the positive or negative reaction after the DNA was amplified and bound on the fluorescence dye, but the fluorescence signal was easily to be affected by the excitation and scattered light. The mini spectrometer was employed to receive the fluorescence intensity in PCR chip in this study, and the optical simulation was progressed, and the stray light shutter (SLS) was added to improve the signal-to-noise ratio (SNR) of fluorescence. The analysis results showed that the SNR of fluorescence can be enhanced from 3.14 to 16.78 by using the SLS with shape of extend component aperture, which the protruding structure was at the direction away from center. The results from this manuscript can provide the important reference information to the developer of qPCR chip, whom can obtain the high SNR fluorescence signal in qPCR inspection process for disease.

原文English
主出版物標題PHOTOPTICS 2021 - Proceedings of the 9th International Conference on Photonics, Optics and Laser Technology
編輯Maria Raposo, Paulo Ribeiro
發行者SciTePress
頁面54-60
頁數7
ISBN(電子)9789897584923
出版狀態Published - 2021
事件9th International Conference on Photonics, Optics and Laser Technology, PHOTOPTICS 2021 - Virtual, Online
持續時間: 2021 二月 112021 二月 13

出版系列

名字PHOTOPTICS 2021 - Proceedings of the 9th International Conference on Photonics, Optics and Laser Technology

Conference

Conference9th International Conference on Photonics, Optics and Laser Technology, PHOTOPTICS 2021
城市Virtual, Online
期間21-02-1121-02-13

All Science Journal Classification (ASJC) codes

  • 原子與分子物理與光學

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