A suction-type microfluidic immunosensing chip for rapid detection of the dengue virus

Chen Hsun Weng, Tze Bin Huang, Chih Chia Huang, Chen Sheng Yeh, Huan Yao Lei, Gwo Bin Lee

研究成果: Article同行評審

24 引文 斯高帕斯(Scopus)

摘要

The enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics. In order to reduce the diagnosis time and to lower the consumption of sample/reagents in an ELISA assay, a suction-type, automatic, pneumatically-driven microfluidic chip has been designed and fabricated in this study. The microfluidic chip integrates a multi-functional micro-transport/ mixing unit, for transporting metering and mixing of samples and reagents in order to automatically perform the entire ELISA protocol. A new surface modification has been adopted which allows for a high processing capacity. The detection sensitivity for the dengue virus is found to be 101 PFU/ml, which is much better than a conventional ELISA assay (103 PFU/ml). The entire assay time is only 30 min, which is much faster than with 96-well microtiter plates (4 h). The consumed sample and reagent volume is only 12 μl, which is less than a conventional assay (100 μl). The development of this microfluidic chip may be promising for other immunosensing applications.

原文English
頁(從 - 到)585-595
頁數11
期刊Biomedical Microdevices
13
發行號3
DOIs
出版狀態Published - 2011 6月

All Science Journal Classification (ASJC) codes

  • 生物醫學工程
  • 分子生物學

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