A technique to enhance electrochemical signals and shorten response time by manipulating yeast near the sensing electrode

Tsung Che Chou, Ching Yu Chang, Kuei Yui Lai, Chi Han Chiou, Chun Hsun Chu, Tomokazu Matsue, Hsien-Chang Chang

研究成果: Conference contribution

摘要

In this work, we manipulated recombinant yeast cells onto a sensing electrode by using hydraulic and electrophoretic force; and thus the current response of trace enzyme activity was increased in p-aminophenyl- β -D - galactopyranoside (PAPG) solution. The recombinant yeast which can generate β-D -galactosidase (β- Gal) in response to steroidal hormone 17 -estradiol were employed as the sensing element of the developed cell-based biosensor. Cells was trapped and stabilized within a microwell structure when they flowed through the sensing electrode. A vertical trapping force was generated by applying +2.0 V across the sensing electrode and an Indium Tin Oxide (ITO). The geometrical electrode area ( 100 × 30 μm2) was defined by a photoresister layer. Since the treated cells were collected on the electrode surface, PAP concentration can be significantly changed around the sensing electrode. Consequently, the detection limit was extend to 0.5 ppb, and the response time was shorten as fast as 15 min by using this device.

原文English
主出版物標題2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
頁面142-145
頁數4
DOIs
出版狀態Published - 2009
事件2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 - Tainan, Taiwan
持續時間: 2009 十月 182009 十月 21

Other

Other2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
國家/地區Taiwan
城市Tainan
期間09-10-1809-10-21

All Science Journal Classification (ASJC) codes

  • 分子醫學
  • 藥理

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