A thermal study on the use of immobilized penicillin G acylase in the formation of 7-amino-3-deacetoxy cephalosporanic acid from cephalosporin G

Jian Liang Pan, Mei-Jywan Syu

研究成果: Article同行評審

4 引文 斯高帕斯(Scopus)

摘要

Penicillin G acylase (PGA) is an important enzyme for the industrial production of 7-amino-3-deacetoxy cephalosporanic acid (7-ADCA) from cephalosporin G (Ceph-G), and 6-aminopenicillanic acid (6-APA) from penicillin G (Pen-G). These products are used for the manufacture of semi-synthetic cephalosporins and penicillins. In this study, immobilized PGA was utilized to catalyze the conversion of Ceph-G to 7-ADCA. The optimal conditions were found to be an operating temperature of 45°C, 0.2 M phosphate buffer, a substrate concentration of 30 mg cm-3 and a catalyst particle concentration of 0.01 g cm-3 (specific activity of 623.2 U g-1). Up to 45°C the reaction was characterized by an activation energy of 38.66 kJ mol-1. Beyond 57.5°C there was a sharp decline of activity, characterized by a deactivation energy of 235.88 kJ mol-1.

原文English
頁(從 - 到)1050-1056
頁數7
期刊Journal of Chemical Technology and Biotechnology
79
發行號10
DOIs
出版狀態Published - 2004 十月 1

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 化學工程 (全部)
  • 可再生能源、永續發展與環境
  • 燃料技術
  • 廢物管理和處置
  • 污染
  • 有機化學
  • 無機化學

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