A validated HPLC method with ultraviolet detection for the determination of buformin in plasma

Chen Hsi Chou, Ching Ling Cheng, Chien Chen Huang

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

A sensitive and rapid high-performance liquid chromatographic assay is developed and validated for the determination of buformin in plasma. After addition of metformin as the internal standard, the analytes were deproteinated with acetonitrile, washed with dichloromethane, and the resulting supernatant injected. Chromatography was performed at ambient temperature by pumping a mobile phase of 0.03 M diammonium hydrogen phosphate buffer (pH 7, 250 mL) in methanol (750 mL) at a flow rate of 1 mL/min through a silica column. Buformin and metformin were detected at 236 nm, and eluted 9.8 and 15.4 min, respectively. No endogenous substances were found to interfere. Calibration curves were linear over the concentration range of 20-2000 ng/mL. The limit of quantitation was 20 ng/mL. The intra- and inter-day relative standard deviation (RSD) was 8.3%, or less, and the accuracy was within 10.1% of the relative error (RE). The method is suitable in pharmacokinetic investigation of buformin.

原文English
頁(從 - 到)254-258
頁數5
期刊Biomedical Chromatography
18
發行號4
DOIs
出版狀態Published - 2004 5月 1

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 生物化學
  • 分子生物學
  • 藥理
  • 藥物發現
  • 臨床生物化學

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