It is critical to set up a precise and feasible monitoring system for a variety of animal or human studies. A behavioral state and physiological signal monitoring system was developed. Four EEG and three-axis accelerometer signals are acquired and transmitted to a host computer for further automatic analysis or visual review. The wireless communication based on IEEE 802.15.4/ZigBee frees the experimental subject from the hassle of wires and reduces the wire artifact of EEG signal during convulsions. This system features low cost, compact size, low power, and real time by eliminating video, EMG and EOG installation and detection. It demonstrates successful observation of kindle process of temporal lobe epilepsy and discrimination vigilance states, while a long period of recording is also verified.