Absence of an Essential Role for Thymic Stromal Lymphopoietin Receptor in Murine B-Cell Development

Nick Carpino, William E. Thierfelder, Ming Shi Chang, Chris Saris, Steven J. Turner, Steven F. Ziegler, James N. Ihle

研究成果: Article

107 引文 (Scopus)

摘要

The murine cytokine thymic stromal lymphopoietin (TSLP) supports the development of B220+ IgM+ immature B cells and induces thymocyte proliferation in vitro. Human TSLP, by contrast, activates CD11c + dendritic cells, but not B or T cells. Recent studies have demonstrated that the receptor for TSLP consists of a heterodimer of the interleukin 7 (IL-7) α chain and a novel protein that resembles the hematopoietic cytokine receptor common γ chain. We examined signal transduction by the γ-like chains using chimeric receptor proteins. The cytoplasmic domain of the human, but not of the murine, γ-like chain, activates Jak2 and Stat5 and supports the proliferation of hematopoietic cell lines. In order to assess the role of the murine γ-like chain in vivo, we generated γ-like chain-deficient mice. Receptor-deficient mice are unresponsive to TSLP but exhibit no obvious phenotypic defects. In particular, hematopoietic cell development appeared normal. B-cell development, including the IgM+ compartment, was unaffected by loss of the TSLP pathway, as were T lymphopoiesis and lymphocyte proliferation in vitro. Cytokine receptors that utilize the common γ chain signal through the lymphocyte-specific kinase Jak3. Mice deficient in Jak3 exhibit a SCID phenotype but harbor a residual B220+ splenic lymphocyte population. We demonstrate here that this residual lymphocyte population is lost in mice lacking both the γ-like chain and Jak3.

原文English
頁(從 - 到)2584-2592
頁數9
期刊Molecular and Cellular Biology
24
發行號6
DOIs
出版狀態Published - 2004 三月 1

指紋

B-Lymphocytes
Lymphocytes
Cytokine Receptors
Immunoglobulin M
Lymphopoiesis
Interleukin-7
B-Lymphoid Precursor Cells
Thymocytes
Dendritic Cells
Population
Signal Transduction
Proteins
Phosphotransferases
thymic stromal lymphopoietin
Cytokines
T-Lymphocytes
Phenotype
Cell Line
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

引用此文

Carpino, Nick ; Thierfelder, William E. ; Chang, Ming Shi ; Saris, Chris ; Turner, Steven J. ; Ziegler, Steven F. ; Ihle, James N. / Absence of an Essential Role for Thymic Stromal Lymphopoietin Receptor in Murine B-Cell Development. 於: Molecular and Cellular Biology. 2004 ; 卷 24, 編號 6. 頁 2584-2592.
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Absence of an Essential Role for Thymic Stromal Lymphopoietin Receptor in Murine B-Cell Development. / Carpino, Nick; Thierfelder, William E.; Chang, Ming Shi; Saris, Chris; Turner, Steven J.; Ziegler, Steven F.; Ihle, James N.

於: Molecular and Cellular Biology, 卷 24, 編號 6, 01.03.2004, p. 2584-2592.

研究成果: Article

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