Activation of tumor suppressor p53 gene expression by magnetic thymine-imprinted chitosan nanoparticles

Mei Hwa Lee, James L. Thomas, Jian Zhou Chen, Jeng Shiung Jan, Hung Yin Lin

研究成果: Article

11 引文 斯高帕斯(Scopus)

摘要

Chitosan is a natural biodegradable polysaccharide that has been used to enhance gene delivery, owing to the ease with which chitosan nanoparticles enter the nucleus of cells. To study the effects of nuclear delivery of telomeric gene sequences, which contain thymine, we formed magnetic thymine-imprinted chitosan nanoparticles (TIPs) by the precipitation of chitosan, mixed with thymine and magnetic nanoparticles (to aid in separations). The mean size of the TIPS was 116 ± 18 nm; the dissociation constant for thymine was 21.8 mg mL-1. We then treated human hepatocellular carcinoma (HepG2) with TIPs nanoparticles bearing bound thymine or a bound telomeric DNA sequence. The expression of the tumor suppressor p53 gene increased when TIPs were applied and decreased when telomere-bound TIPs were applied.

原文English
頁(從 - 到)2137-2140
頁數4
期刊Chemical Communications
52
發行號10
DOIs
出版狀態Published - 2016 一月 1

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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