Agmatine Induces Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2-Receptors

Liang Ming Lee, Chia Ho Lin, Hsien Hui Chung, Juei Tang Cheng, I. Hung Chen, Yat Ching Tong

研究成果: Article同行評審

4 引文 斯高帕斯(Scopus)

摘要

Objectives: The effect of agmatine on prostate contractility as well as the roles of imidazoline receptors and potassium channels in this action were studied using isolated Wistar rat prostate tissue. Methods: Rat prostate strips were pre-contracted with 1 μmol/L phenylephrine or 50 mmol/L KCl. The relaxation response to agmatine (1-100 μmol/L) was measured. The effects of imidazoline receptor blockers: efaroxan, BU224, KU14R; ATP-sensitive K+ channels (KATP) channel inhibitor: glibenclamide; cyclic AMP (cAMP) phosphodiesterase inhibitor: IBMX; or protein kinase A (PKA) inhibitor: H-89 on the agmatine-induced relaxation were studied. Results: Agmatine produced relaxation in prostate strips pre-contracted with phenylephrine or KCl in a dose-dependent manner. This relaxation was significantly reduced by BU224, a selective I2 imidazoline receptor (IR) blocker, but not by I1 or I3 IR blockers (efaroxan, KU14R respectively). Moreover, the agmatine-induced relaxation was attenuated by glibenclamide and H-89, but enhanced by IBMX. Conclusion: The results suggest that agmatine causes rat prostate relaxation by activation of the I2 IR, which opens KATP channels through cAMP/PKA pathway.

原文English
頁(從 - 到)39-43
頁數5
期刊LUTS: Lower Urinary Tract Symptoms
5
發行號1
DOIs
出版狀態Published - 2013 一月

All Science Journal Classification (ASJC) codes

  • Neurology
  • Urology

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