Algorithmic robustness to preferred orientations in single particle analysis by CryoEM

C. O.S. Sorzano, D. Semchonok, S. C. Lin, Y. C. Lo, J. L. Vilas, A. Jiménez-Moreno, M. Gragera, S. Vacca, D. Maluenda, M. Martínez, E. Ramírez-Aportela, R. Melero, A. Cuervo, J. J. Conesa, P. Conesa, P. Losana, L. del Caño, J. Jiménez de la Morena, Y. C. Fonseca, R. Sánchez-GarcíaD. Strelak, E. Fernández-Giménez, F. de Isidro, D. Herreros, P. L. Kastritis, R. Marabini, B. D. Bruce, J. M. Carazo

研究成果: Article同行評審

摘要

The presence of preferred orientations in single particle analysis (SPA) by cryo-Electron Microscopy (cryoEM) is currently one of the hurdles preventing many structural analyses from yielding high-resolution structures. Although the existence of preferred orientations is mostly related to the grid preparation, in this technical note, we show that some image processing algorithms used for angular assignment and three-dimensional (3D) reconstruction are more robust than others to these detrimental conditions. We exemplify this argument with three different data sets in which the presence of preferred orientations hindered achieving a 3D reconstruction without artifacts or, even worse, a 3D reconstruction could never be achieved.

原文English
文章編號107695
期刊Journal of Structural Biology
213
發行號1
DOIs
出版狀態Published - 2021 三月

All Science Journal Classification (ASJC) codes

  • 結構生物學

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