Alpha 1-acid glycoprotein-induced tumor necrosis factor-α secretion of human monocytes is enhanced by serum binding proteins and depends on protein tyrosine kinase activation

Shu Jem Su, Bei Chang Yang, Yuh Sheng Wang, Trai Ming Yeh

研究成果: Article同行評審

19 引文 斯高帕斯(Scopus)

摘要

The acute phase protein, α1 acid glycoprotein (AGP), stimulated human mononuclear cells as well as monocytes to secrete tumor necrosis factor-α (TNFα) which was demonstrated by ELISA, RT-PCR and functional assays. AGP-induced TNFα secretion of monocytes was enhanced in the presence of human plasma and inhibited by protein kinase inhibitors, indicating it is serum and tyrosine kinase dependent. The activation of tyrosine kinase in AGP-stimulated monocytes was further confirmed by immunoblotting of tyrosine phosphorylated proteins of monocytes at different time after AGP stimulation. Furthermore, several serum proteins such as C3, sCD14 and IgG were able to bind to AGP and enhanced TNFα secretion of human monocytes induced by AGP. Taken together, these results suggest serum proteins binding to AGP enhance its ability to stimulate human monocytes to secrete pro-inflammatory cytokines through a tyrosine kinase dependent pathway. Copyright (C) 1999 Elsevier Science B.V.

原文English
頁(從 - 到)21-29
頁數9
期刊Immunopharmacology
41
發行號1
DOIs
出版狀態Published - 1999 一月

All Science Journal Classification (ASJC) codes

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