Alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp Litopenaeus vannamai

Sirikwan Ponprateep, Tipachai Vatanavicharn, Chu Fang Lo, Anchalee Tassanakajon, Vichien Rimphanitchayakit

研究成果: Article同行評審

29 引文 斯高帕斯(Scopus)

摘要

The shrimp multifunctional protein alpha-2-macroglobulin (A2M) is abundantly expressed in plasma, highly up-regulated upon microbial infection and involved in several immune pathways such as blood clotting system, phagocytosis and melanization. Herein, the function of LvA2M from Litopenaeus vannamei on the prophenoloxidase (proPO) system is reported. The recombinant (r)LvA2M produced strongly and specifically inhibited trypsin and the PO activity in shrimp plasma in a dose-dependent manner. Silencing of LvA2M led to an increase in the PO activity in shrimp plasma although the expression of proPO-associated genes, proPO-activating enzyme (PPAE) and prophenoloxidase (proPO) but not the proPO-activating factor (PPAF) was down-regulated. In Vibrio parahaemolyticus AHPND-infected shrimp, the LvA2M activity was suppressed in an early phase of infection while the PO activity was increased. Thus, the proPO-activating system was regulated by the LvA2M.

原文English
頁(從 - 到)68-74
頁數7
期刊Fish and Shellfish Immunology
62
DOIs
出版狀態Published - 2017 三月 1

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

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