Altered mitochondrial atp synthase expression in the rat dorsal root ganglion after sciatic nerve injury and analgesic effects of intrathecal ATP

Kuan Hung Chen, Chung Ren Lin, Jiin Tsuey Cheng, Jen Kun Cheng, Wen Tzu Liao, Chien Hui Yang

研究成果: Article同行評審

8 引文 斯高帕斯(Scopus)

摘要

Mitochondrial ATP synthase has multiple interdependent biological functions in neurons. Among them, ATP generation and regulation are the most important. The present study investigated whether the expression of mitochondrial ATP synthase correlates with symptoms of neuropathic pain in adult rats after axotomy, and whether intrathecal ATP administration is therapeutic in these neuropathic rats. Male Sprague-Dawley rats received left sciatic nerve transection (axotomy) and were randomly designated to a control (sham-operated) group, a neuropathic pain group (axotomy), a neuropathic pain and intrathecal sterile saline group, and a neuropathic pain and intrathecal ATP group. The thermal and mechanical sensitivity tests were performed at 1, 3, 5, and 7 days after axotomy. Left L4-L5 dorsal root ganglions (DRGs) were harvested to assess mitochondrial ATP synthase by immunoblotting and immunohistochemistry. After nerve injury, the expression of mitochondrial ATP synthase was decreased in protein extracts and was found mainly in C-fiber and A-δ fiber neurons of the DRGs. The decreased expression of mitochondrial ATP synthase and its subcellular localization were related to thermal and mechanical hyperalgesia. Administration of intrathecal ATP significantly attenuated thermal and mechanical hypersensitivity throughout the experimental period, which suggests its potential role in the treatment of neuropathic pain.

原文English
頁(從 - 到)51-59
頁數9
期刊Cellular and Molecular Neurobiology
34
發行號1
DOIs
出版狀態Published - 2014 一月

All Science Journal Classification (ASJC) codes

  • 細胞與分子神經科學
  • 細胞生物學

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