Altered neuronatin expression in the rat dorsal root ganglion after sciatic nerve transection

Kuan Hung Chen, Chien Hui Yang, Jiin Tsuey Cheng, Chih Hsien Wu, Wei Dih Sy, Chung Ren Lin

研究成果: Article同行評審

9 引文 斯高帕斯(Scopus)

摘要

Background. Several molecular changes occur following axotomy, such as gene up-regulation and down-regulation. In our previous study using Affymetrix arrays, it was found that after the axotomy of sciatic nerve, there were many novel genes with significant expression changes. Among them, neuronatin (Nnat) was the one which expression was significantly up-regulated. Nnat was identified as a gene selectively expressed in neonatal brains and markedly reduced in adult brains. The present study investigated whether the expression of Nnat correlates with symptoms of neuropathic pain in adult rats with transected sciatic nerve. Methods. Western blotting, immunohistochemistry, and the Randall and Selitto test were used to study the protein content, and subcellular localization of Nnat in correlation with pain-related animal behavior. Results. It was found that after nerve injury, the expression of Nnat was increased in total protein extracts. Unmyelinated C-fiber and thinly myelinated A- fiber in adult dorsal root ganglions (DRGs) were the principal sub-population of primary afferent neurons with distributed Nnat. The increased expression of Nnat and its subcellular localization were related to mechanical hyperalgesia. Conclusions. The results indicated that there was significant correlation between mechanical hyperalgesia in axotomy of sciatic nerve and the increased expression of Nnat in C-fiber and A- fiber of adult DRG neurons.

原文English
文章編號41
期刊Journal of biomedical science
17
發行號1
DOIs
出版狀態Published - 2010

All Science Journal Classification (ASJC) codes

  • 內分泌學、糖尿病和代謝
  • 分子生物學
  • 臨床生物化學
  • 細胞生物學
  • 生物化學(醫學)
  • 藥學(醫學)

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