An alternative method to quantify 2-MIB producing cyanobacteria in drinking water reservoirs: Method development and field applications

Yi Ting Chiu, Hung Kai Yen, Tsair Fuh Lin

研究成果: Article同行評審

17 引文 斯高帕斯(Scopus)

摘要

2-Methylisoborneol (2-MIB) is a commonly detected cyanobacterial odorant in drinking water sources in many countries. To provide safe and high-quality water, development of a monitoring method for the detection of 2-MIB-synthesis (mibC) genes is very important. In this study, new primers MIBS02F/R intended specifically for the mibC gene were developed and tested. Experimental results show that the MIBS02F/R primer set was able to capture 13 2-MIB producing cyanobacterial strains grown in the laboratory, and to effectively amplify the targeted DNA region from 17 2-MIB-producing cyanobacterial strains listed in the literature. The primers were further coupled with a TaqMan probe to detect 2-MIB producers in 29 drinking water reservoirs (DWRs). The results showed statistically significant correlations between mibC genes and 2-MIB concentrations for the data from each reservoir (R2=0.413–0.998; p<0.05), from all reservoirs in each of the three islands (R2=0.302–0.796; p<0.01), and from all data of the three islands (R2=0.473–0.479; p<0.01). The results demonstrate that the real-time PCR can be an alternative method to provide information to managers of reservoirs and water utilities facing 2-MIB-related incidents.

原文English
頁(從 - 到)618-627
頁數10
期刊Environmental Research
151
DOIs
出版狀態Published - 2016 十一月 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Environmental Science(all)

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