An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System

Anirban Sinha, Priya Gopinathan, Yi Da Chung, Shu Chu Shiesh, Gwo Bin Lee

研究成果: Conference contribution

摘要

Cardiovascular diseases (CVDs) have resulted in an estimated death toll of nearly 17 million yearly. Early detection of CVDs may reduce the risk of sudden deaths. The current technologies based on antigen-antibody interactions have limitations in point-of-care (POC) applications. This study therefore reports an aptamer magnetic bead-based assay which is relatively sensitive and highly selective on a new integrated microfluidic platform for simultaneous analysis of up to six untreated clinical serum samples utilizing less than a volume of 10 μL from each patient. Experimental results showed that one of the important biomarkers for CVDs, cardiac troponin I, could be detected on the microfluidic platform in less than 35 minutes with a limit of detection of 0.01 ng/mL. It may provide a promising device for POC applications.

原文English
主出版物標題2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
發行者Institute of Electrical and Electronics Engineers Inc.
頁面1075-1077
頁數3
ISBN(電子)9781728120072
DOIs
出版狀態Published - 2019 六月
事件20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany
持續時間: 2019 六月 232019 六月 27

出版系列

名字2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
國家Germany
城市Berlin
期間19-06-2319-06-27

指紋

biomarkers
Sandwich
Microfluidics
Biomarkers
Assays
death
platforms
Sudden Death
Troponin I
antigens
Antigens
antibodies
Antibody
Antibodies
beads
Cardiac
serums
Experimental Results
Interaction
interactions

All Science Journal Classification (ASJC) codes

  • Process Chemistry and Technology
  • Spectroscopy
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Control and Optimization
  • Instrumentation

引用此文

Sinha, A., Gopinathan, P., Chung, Y. D., Shiesh, S. C., & Lee, G. B. (2019). An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. 於 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (頁 1075-1077). [8808551] (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808551
Sinha, Anirban ; Gopinathan, Priya ; Chung, Yi Da ; Shiesh, Shu Chu ; Lee, Gwo Bin. / An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. 頁 1075-1077 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).
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abstract = "Cardiovascular diseases (CVDs) have resulted in an estimated death toll of nearly 17 million yearly. Early detection of CVDs may reduce the risk of sudden deaths. The current technologies based on antigen-antibody interactions have limitations in point-of-care (POC) applications. This study therefore reports an aptamer magnetic bead-based assay which is relatively sensitive and highly selective on a new integrated microfluidic platform for simultaneous analysis of up to six untreated clinical serum samples utilizing less than a volume of 10 μL from each patient. Experimental results showed that one of the important biomarkers for CVDs, cardiac troponin I, could be detected on the microfluidic platform in less than 35 minutes with a limit of detection of 0.01 ng/mL. It may provide a promising device for POC applications.",
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Sinha, A, Gopinathan, P, Chung, YD, Shiesh, SC & Lee, GB 2019, An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. 於 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808551, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., 頁 1075-1077, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 19-06-23. https://doi.org/10.1109/TRANSDUCERS.2019.8808551

An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. / Sinha, Anirban; Gopinathan, Priya; Chung, Yi Da; Shiesh, Shu Chu; Lee, Gwo Bin.

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 1075-1077 8808551 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

研究成果: Conference contribution

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AU - Gopinathan, Priya

AU - Chung, Yi Da

AU - Shiesh, Shu Chu

AU - Lee, Gwo Bin

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AB - Cardiovascular diseases (CVDs) have resulted in an estimated death toll of nearly 17 million yearly. Early detection of CVDs may reduce the risk of sudden deaths. The current technologies based on antigen-antibody interactions have limitations in point-of-care (POC) applications. This study therefore reports an aptamer magnetic bead-based assay which is relatively sensitive and highly selective on a new integrated microfluidic platform for simultaneous analysis of up to six untreated clinical serum samples utilizing less than a volume of 10 μL from each patient. Experimental results showed that one of the important biomarkers for CVDs, cardiac troponin I, could be detected on the microfluidic platform in less than 35 minutes with a limit of detection of 0.01 ng/mL. It may provide a promising device for POC applications.

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Sinha A, Gopinathan P, Chung YD, Shiesh SC, Lee GB. An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. 於 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 1075-1077. 8808551. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). https://doi.org/10.1109/TRANSDUCERS.2019.8808551