摘要
Cardiovascular diseases (CVDs) have resulted in an estimated death toll of nearly 17 million yearly. Early detection of CVDs may reduce the risk of sudden deaths. The current technologies based on antigen-antibody interactions have limitations in point-of-care (POC) applications. This study therefore reports an aptamer magnetic bead-based assay which is relatively sensitive and highly selective on a new integrated microfluidic platform for simultaneous analysis of up to six untreated clinical serum samples utilizing less than a volume of 10 μL from each patient. Experimental results showed that one of the important biomarkers for CVDs, cardiac troponin I, could be detected on the microfluidic platform in less than 35 minutes with a limit of detection of 0.01 ng/mL. It may provide a promising device for POC applications.
| 原文 | English |
|---|---|
| 主出版物標題 | 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII |
| 發行者 | Institute of Electrical and Electronics Engineers Inc. |
| 頁面 | 1075-1077 |
| 頁數 | 3 |
| ISBN（電子） | 9781728120072 |
| DOIs | |
| 出版狀態 | Published - 2019 六月 |
| 事件 | 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany 持續時間: 2019 六月 23 → 2019 六月 27 |
出版系列
| 名字 | 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII |
|---|
Conference
| Conference | 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII |
|---|---|
| 國家 | Germany |
| 城市 | Berlin |
| 期間 | 19-06-23 → 19-06-27 |
指紋
All Science Journal Classification (ASJC) codes
- Process Chemistry and Technology
- Spectroscopy
- Electrical and Electronic Engineering
- Mechanical Engineering
- Electronic, Optical and Magnetic Materials
- Control and Optimization
- Instrumentation
引用此文
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An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System. / Sinha, Anirban; Gopinathan, Priya; Chung, Yi Da; Shiesh, Shu Chu; Lee, Gwo Bin.
2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 1075-1077 8808551 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).研究成果: Conference contribution
TY - GEN
T1 - An Aptamer Based Sandwich Assay for Simultaneous Detection of Multiple Cardiovascular Biomarkers on A Multilayered Integrated Microfluidic System
AU - Sinha, Anirban
AU - Gopinathan, Priya
AU - Chung, Yi Da
AU - Shiesh, Shu Chu
AU - Lee, Gwo Bin
PY - 2019/6
Y1 - 2019/6
N2 - Cardiovascular diseases (CVDs) have resulted in an estimated death toll of nearly 17 million yearly. Early detection of CVDs may reduce the risk of sudden deaths. The current technologies based on antigen-antibody interactions have limitations in point-of-care (POC) applications. This study therefore reports an aptamer magnetic bead-based assay which is relatively sensitive and highly selective on a new integrated microfluidic platform for simultaneous analysis of up to six untreated clinical serum samples utilizing less than a volume of 10 μL from each patient. Experimental results showed that one of the important biomarkers for CVDs, cardiac troponin I, could be detected on the microfluidic platform in less than 35 minutes with a limit of detection of 0.01 ng/mL. It may provide a promising device for POC applications.
AB - Cardiovascular diseases (CVDs) have resulted in an estimated death toll of nearly 17 million yearly. Early detection of CVDs may reduce the risk of sudden deaths. The current technologies based on antigen-antibody interactions have limitations in point-of-care (POC) applications. This study therefore reports an aptamer magnetic bead-based assay which is relatively sensitive and highly selective on a new integrated microfluidic platform for simultaneous analysis of up to six untreated clinical serum samples utilizing less than a volume of 10 μL from each patient. Experimental results showed that one of the important biomarkers for CVDs, cardiac troponin I, could be detected on the microfluidic platform in less than 35 minutes with a limit of detection of 0.01 ng/mL. It may provide a promising device for POC applications.
UR - http://www.scopus.com/inward/record.url?scp=85071918061&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071918061&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2019.8808551
DO - 10.1109/TRANSDUCERS.2019.8808551
M3 - Conference contribution
AN - SCOPUS:85071918061
T3 - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
SP - 1075
EP - 1077
BT - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
PB - Institute of Electrical and Electronics Engineers Inc.
ER -