An artificial cilia-based micromixer towards the activation of zebrafish sperms

Pin Yi Huang, Bivas Panigrahi, Chang Hung Lu, Ping Feng Huang, Chia-Yuan Chen

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

Zebrafish sperm activation is a necessary step in zebrafish research to satisfy large demands for current vertebral experiments, and the success of sperm motility is significantly affected by the magnitude of fluid-induced shear force. In traditional methods, zebrafish sperms are activated by rapidly diluting them in an aqueous medium through manual mixing. However, this method is inefficient because of low activation rates. This study is aimed to develop an artificial cilia-based micromixer through the precise regulation of hydrodynamics induced on zebrafish sperms for superior sperm activation. Results revealed that the proposed device activated as high as 68% of the tested sperms compared with an average of 44% sperms activated through the manual mixing. Therefore, sperm activation was significantly improved by 50% (p < 0.05). Parameters such as artificial rotation frequency and artificial cilia actuation time period were further evaluated and characterized. Zebrafish sperms were found to be activated within 5 s by using the proposed device. Thus, the proposed micromixer is envisaged that can advance the studies related to sperm dynamics and in-vitro fertilization procedures for vertebrate.

原文English
頁(從 - 到)541-548
頁數8
期刊Sensors and Actuators, B: Chemical
244
DOIs
出版狀態Published - 2017 一月 1

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 儀器
  • 凝聚態物理學
  • 表面、塗料和薄膜
  • 金屬和合金
  • 電氣與電子工程
  • 材料化學

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