An automatic microfluidic system for continuous selection of aptamers targeting surface protein by using cancer tissue samples

Wei Ting Liu, Yi Cheng Tsai, Wen Bin Lee, Chien Yu Fu, Yuan Jhe Chuang, Keng Fu Hsu, Gwo Bin Lee

研究成果: Conference contribution

摘要

Aptamers have been regarded as promising artificial antibodies with high stability. To successfully select aptamers that were able to be directedly adopted for clinical applications, we have developed an integrated microfluidic chip to perform a tissue-based SELEX (tissue-SELEX) in which time and processes of SELEX were reduced and simplified. However, our previous design required a large number of tissue specimen and chips fabricated for individual rounds of SELEX. Moreover, many aptamers targeting cytosolic or nucleus proteins were selected through tissue sections that could not be used in membrane-specific target therapy and diagnosis. In this work, we reported the first automatic microfluidic system which was capable of performing "continuous" SELEX with clinical "tissue" samples, which only took 12 hours to complete 6 rounds of SELEX with minimum volume of tissue samples and reagents. In addition, in order to further improve the selection outcome, a single-stranded DNA (ssDNA) library specific to the cell membrane was pre-selected such that the screened aptamers could be specifically bounded on the cell surfaces for targeting and diagnostic applications.

原文English
主出版物標題22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
發行者Chemical and Biological Microsystems Society
頁面1910-1913
頁數4
ISBN(電子)9781510897571
出版狀態Published - 2018 一月 1
事件22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
持續時間: 2018 十一月 112018 十一月 15

出版系列

名字22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
4

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
國家Taiwan
城市Kaohsiung
期間18-11-1118-11-15

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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  • 引用此

    Liu, W. T., Tsai, Y. C., Lee, W. B., Fu, C. Y., Chuang, Y. J., Hsu, K. F., & Lee, G. B. (2018). An automatic microfluidic system for continuous selection of aptamers targeting surface protein by using cancer tissue samples. 於 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (頁 1910-1913). (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018; 卷 4). Chemical and Biological Microsystems Society.