An echovirus 18-associated outbreak of aseptic meningitis in Taiwan: Epidemiology and diagnostic and genetic aspects

Huey Pin Tsai, Sheng Wen Huang, Feng Ling Wu, Pin Hwa Kuo, Shih Min Wang, Ching Chuan Liu, Ih Jen Su, Jen Ren Wang

研究成果: Article

15 引文 (Scopus)

摘要

In 2006, an outbreak of aseptic meningitis was noted in Taiwan. From January to October 2006, a total of 3283 specimens collected from patients with viral infection, including 173 cerebrospinal fluid (CSF) samples, were examined for virus isolation and identification. Overall, 339 enterovirus (EV)-positive cases were identified by virus culture: echovirus 18 (E18) formed the majority (27.4%, 93 cases), followed by coxsackievirus B2 (13.8%, 47 cases) and coxsackievirus A2 (10.8%, 37 cases). The manifestations of the 93 E18 cases were aseptic meningitis (44.1%), viral exanthema (23.6%), acute tonsillitis (15.1%), acute pharyngitis (14.0%), acute gastritis (11.8%), herpangina (7.5%) and bronchopneumonia (5.3%). Of 107 E18 isolates identified, 100, 62.5 and 19% were obtained following culture in RD, MRC-5 and A549 cells, respectively. E18 was identified most frequently from throat swabs (67.2%) and less frequently from stool samples (15.9%) and CSF (16.8%). The detection rate of E18 was 78.2% from CSF, 50% from stool samples and 22.9% from throat swabs. Phylogenetic relationships among the E18 strains were examined. Analysis of the partial VP1 gene showed 3.7-23.8% variation in sequence compared with sequences from GenBank and, notably, the amino acid change V152S was detected in a protruding loop within the VP1 protein. These results indicate that a genetic variant of E18 was circulating and caused an outbreak of aseptic meningitis in Taiwan in 2006.

原文English
頁(從 - 到)1360-1365
頁數6
期刊Journal of Medical Microbiology
60
發行號9
DOIs
出版狀態Published - 2011 九月 1

指紋

Aseptic Meningitis
Human Enterovirus B
Molecular Epidemiology
Taiwan
Disease Outbreaks
Enterovirus
Cerebrospinal Fluid
Pharynx
Herpangina
Viruses
Bronchopneumonia
Tonsillitis
Pharyngitis
Nucleic Acid Databases
Gastritis
Virus Diseases
Exanthema
varespladib methyl
Amino Acids

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

引用此文

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title = "An echovirus 18-associated outbreak of aseptic meningitis in Taiwan: Epidemiology and diagnostic and genetic aspects",
abstract = "In 2006, an outbreak of aseptic meningitis was noted in Taiwan. From January to October 2006, a total of 3283 specimens collected from patients with viral infection, including 173 cerebrospinal fluid (CSF) samples, were examined for virus isolation and identification. Overall, 339 enterovirus (EV)-positive cases were identified by virus culture: echovirus 18 (E18) formed the majority (27.4{\%}, 93 cases), followed by coxsackievirus B2 (13.8{\%}, 47 cases) and coxsackievirus A2 (10.8{\%}, 37 cases). The manifestations of the 93 E18 cases were aseptic meningitis (44.1{\%}), viral exanthema (23.6{\%}), acute tonsillitis (15.1{\%}), acute pharyngitis (14.0{\%}), acute gastritis (11.8{\%}), herpangina (7.5{\%}) and bronchopneumonia (5.3{\%}). Of 107 E18 isolates identified, 100, 62.5 and 19{\%} were obtained following culture in RD, MRC-5 and A549 cells, respectively. E18 was identified most frequently from throat swabs (67.2{\%}) and less frequently from stool samples (15.9{\%}) and CSF (16.8{\%}). The detection rate of E18 was 78.2{\%} from CSF, 50{\%} from stool samples and 22.9{\%} from throat swabs. Phylogenetic relationships among the E18 strains were examined. Analysis of the partial VP1 gene showed 3.7-23.8{\%} variation in sequence compared with sequences from GenBank and, notably, the amino acid change V152S was detected in a protruding loop within the VP1 protein. These results indicate that a genetic variant of E18 was circulating and caused an outbreak of aseptic meningitis in Taiwan in 2006.",
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An echovirus 18-associated outbreak of aseptic meningitis in Taiwan : Epidemiology and diagnostic and genetic aspects. / Tsai, Huey Pin; Huang, Sheng Wen; Wu, Feng Ling; Kuo, Pin Hwa; Wang, Shih Min; Liu, Ching Chuan; Su, Ih Jen; Wang, Jen Ren.

於: Journal of Medical Microbiology, 卷 60, 編號 9, 01.09.2011, p. 1360-1365.

研究成果: Article

TY - JOUR

T1 - An echovirus 18-associated outbreak of aseptic meningitis in Taiwan

T2 - Epidemiology and diagnostic and genetic aspects

AU - Tsai, Huey Pin

AU - Huang, Sheng Wen

AU - Wu, Feng Ling

AU - Kuo, Pin Hwa

AU - Wang, Shih Min

AU - Liu, Ching Chuan

AU - Su, Ih Jen

AU - Wang, Jen Ren

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