An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

V. Chia Hsuan Tseng, Chee Ho Chew, Wan Ting Huang, Yang Kao Wang, Ko Shao Chen, Szu Yi Chou, Chien Chung Chen

研究成果: Article

3 引文 (Scopus)

摘要

Recently, a novel substrate known as an electrospun polylactic acid (PLLA) microtube array membrane (MTAM) was successfully developed as a cell coculture platform. Structurally, this substrate is made up of one-to-one connected, ultrathin, submicron scale fibers that are arranged in an arrayed formation. Its unique structure confers several key advantages which are beneficial in a cell coculture system. In this study, the interaction between rat fetal neural stem cells (NSC) and astrocytes was examined by comparing the outcome of a typical Transwell-based coculture system and that of an electrospun PLLA MTAM-based coculture system. Compared to tissue culture polystyrene (TCP) and Transwell coculture inserts, a superior cell viability of NSC was observed when cultured in lumens of electrospun PLLA MTAM (with supportive immunostaining images). Reverse transcription polymerase chain reaction revealed a strong interaction between astrocytes and NSC through a higher expression of doublecortin and a lower expression of nestin. These data demonstrate that MTAM is clearly a better coculture platform than the traditional Transwell system.

原文English
頁(從 - 到)179-190
頁數12
期刊Cells Tissues Organs
204
發行號3-4
DOIs
出版狀態Published - 2017 十月 1

指紋

Nerve Regeneration
Coculture Techniques
Membranes
Neural Stem Cells
Astrocytes
Fetal Stem Cells
Nestin
Polystyrenes
Reverse Transcription
Cell Survival
Polymerase Chain Reaction
poly(lactic acid)

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

引用此文

Tseng, V. Chia Hsuan ; Chew, Chee Ho ; Huang, Wan Ting ; Wang, Yang Kao ; Chen, Ko Shao ; Chou, Szu Yi ; Chen, Chien Chung. / An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration. 於: Cells Tissues Organs. 2017 ; 卷 204, 編號 3-4. 頁 179-190.
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An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration. / Tseng, V. Chia Hsuan; Chew, Chee Ho; Huang, Wan Ting; Wang, Yang Kao; Chen, Ko Shao; Chou, Szu Yi; Chen, Chien Chung.

於: Cells Tissues Organs, 卷 204, 編號 3-4, 01.10.2017, p. 179-190.

研究成果: Article

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AU - Chen, Ko Shao

AU - Chou, Szu Yi

AU - Chen, Chien Chung

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