An electro-microchip for immunoassay using gold nanoparticle and silver enhancement

Keng Shiang Huang; Hao Hsuan Huang; Tsung Chain Chang; Hong Ping Lin; Yung Chiang Chung; Yu Cheng Lin

An electro-microchip for immunoassay using gold nanoparticle and silver enhancement

Keng Shiang Huang, Hao Hsuan Huang, Tsung Chain Chang, Hong Ping Lin, Yung Chiang Chung, Yu Cheng Lin

研究成果: Paper › 同行評審

摘要

This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, gold nanoparticles (ANPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The results show that using the gap size of 20 μm electro-chip has the best sensitivity. The relationship between concentration and signal is established and the antigen detection limit is 100 ng/mL.

原文	English
頁面	305-307
頁數	3
出版狀態	Published - 2006 一月 1
事件	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan 持續時間: 2006 十一月 5 → 2006 十一月 9

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
國家	Japan
城市	Tokyo
期間	06-11-05 → 06-11-09

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

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引用此

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title = "An electro-microchip for immunoassay using gold nanoparticle and silver enhancement",

abstract = "This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, gold nanoparticles (ANPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The results show that using the gap size of 20 μm electro-chip has the best sensitivity. The relationship between concentration and signal is established and the antigen detection limit is 100 ng/mL.",

author = "Huang, {Keng Shiang} and Huang, {Hao Hsuan} and Chang, {Tsung Chain} and Lin, {Hong Ping} and Chung, {Yung Chiang} and Lin, {Yu Cheng}",

year = "2006",

month = jan,

day = "1",

language = "English",

pages = "305--307",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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An electro-microchip for immunoassay using gold nanoparticle and silver enhancement. / Huang, Keng Shiang; Huang, Hao Hsuan; Chang, Tsung Chain; Lin, Hong Ping; Chung, Yung Chiang; Lin, Yu Cheng.

2006. 305-307 論文發表於 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

研究成果: Paper › 同行評審

TY - CONF

T1 - An electro-microchip for immunoassay using gold nanoparticle and silver enhancement

AU - Huang, Keng Shiang

AU - Huang, Hao Hsuan

AU - Chang, Tsung Chain

AU - Lin, Hong Ping

AU - Chung, Yung Chiang

AU - Lin, Yu Cheng

PY - 2006/1/1

Y1 - 2006/1/1

N2 - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, gold nanoparticles (ANPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The results show that using the gap size of 20 μm electro-chip has the best sensitivity. The relationship between concentration and signal is established and the antigen detection limit is 100 ng/mL.

AB - This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, gold nanoparticles (ANPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The results show that using the gap size of 20 μm electro-chip has the best sensitivity. The relationship between concentration and signal is established and the antigen detection limit is 100 ng/mL.

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T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

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