This paper reports a novel immunoassay using an electro-microchip to detect the immuno-reaction signal and the silver enhancement reaction to magnify the detection signal. Moreover, gold nanoparticles (ANPs) play the roles of a label for antigen or antibody and a catalyst for silver precipitation. The change of the impedance to corroborate immuno-reaction is detected, amplified and analyzed. The results show that using the gap size of 20 μm electro-chip has the best sensitivity. The relationship between concentration and signal is established and the antigen detection limit is 100 ng/mL.
|出版狀態||Published - 2006 一月 1|
|事件||10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan|
持續時間: 2006 十一月 5 → 2006 十一月 9
|Other||10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006|
|期間||06-11-05 → 06-11-09|
All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)