An electrochemical tyrosinase-immobilized biosensor for albumin - Toward a potential total protein measurement

Min Chieh Chuang, Chung Chiun Liu, Ming Chang Yang

研究成果: Article同行評審

14 引文 斯高帕斯(Scopus)

摘要

A simple biosensor, based on a tyrosinase-immobilized electrode, was developed for a rapid and quantitative measurement of bovine serum albumin (BSA) and human serum albumin (HSA). Tyrosinase, immobilized on a screen-printed carbon electrode, catalyzed the oxidation of tyrosine residues in the albumin to the corresponding quinone, which was further reduced electrochemically by the sensing electrode under an appropriate condition. The concentration of the protein, therefore, could be quantified by measuring the reduction current. The operational parameters that affect the performance of this biosensor, e.g., the working potential, the pH value, and the temperature, were assessed and optimized. The stability, the lifetime, and the reproducibility of this biosensor were also evaluated. This biosensor demonstrated that it was not only a highly sensitive assay for albumin but will be a potential tool for the measurement of total protein in human serum.

原文English
頁(從 - 到)357-363
頁數7
期刊Sensors and Actuators, B: Chemical
114
發行號1
DOIs
出版狀態Published - 2006 三月 30

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 儀器
  • 凝聚態物理學
  • 表面、塗料和薄膜
  • 金屬和合金
  • 電氣與電子工程
  • 材料化學

指紋

深入研究「An electrochemical tyrosinase-immobilized biosensor for albumin - Toward a potential total protein measurement」主題。共同形成了獨特的指紋。

引用此