An in vitro monitoring system for simulated thermal process in cryosurgery

研究成果: Article

15 引文 (Scopus)

摘要

This paper describes a new in vitro experimental model that records temperature changes over a culture plate, which then can be used to assess the biological effects of cryosurgery. A cryoprobe and 16 thermocouples set up by a computer control system were used to monitor the temperature changes during freezing and thawing in a culture plate, and the data were used to create a temperature profile of the entire plate. Location of the thermocouples was confirmed by a digital camera viewing from under the plate, and temperature changes at any point in the interpolated areas were estimated using a curve fitting method. The estimated temperature was checked by sampling with four additional randomly placed thermocouples. Linear regression analysis showed that the estimated temperature and measured temperature were very close (correlation coefficients 0.98-0.99). MBT-2 tumor cells were cultured and then subjected to simulated cryosurgery. The surviving cells were stained with crystal violet and the cell death boundary was detected by image processing. Temperature history at the cell death boundary was retrieved and analyzed. With this system it is possible to recreate the temperature changes that result in a certain biological effect (such as cell death) during the process of simulated cryosurgery. (C) 2000 Academic Press.

原文English
頁(從 - 到)159-170
頁數12
期刊Cryobiology
40
發行號2
DOIs
出版狀態Published - 2000 一月 1

指紋

Cryosurgery
cryosurgery
Hot Temperature
heat
Temperature
Monitoring
monitoring
thermocouples
temperature
Cell death
Thermocouples
cell death
Cell Death
gentian violet
Cultured Tumor Cells
Gentian Violet
In Vitro Techniques
Computer control systems
Thawing
Computer Systems

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

引用此文

@article{c441857a5dcd45658e1798187f8d444d,
title = "An in vitro monitoring system for simulated thermal process in cryosurgery",
abstract = "This paper describes a new in vitro experimental model that records temperature changes over a culture plate, which then can be used to assess the biological effects of cryosurgery. A cryoprobe and 16 thermocouples set up by a computer control system were used to monitor the temperature changes during freezing and thawing in a culture plate, and the data were used to create a temperature profile of the entire plate. Location of the thermocouples was confirmed by a digital camera viewing from under the plate, and temperature changes at any point in the interpolated areas were estimated using a curve fitting method. The estimated temperature was checked by sampling with four additional randomly placed thermocouples. Linear regression analysis showed that the estimated temperature and measured temperature were very close (correlation coefficients 0.98-0.99). MBT-2 tumor cells were cultured and then subjected to simulated cryosurgery. The surviving cells were stained with crystal violet and the cell death boundary was detected by image processing. Temperature history at the cell death boundary was retrieved and analyzed. With this system it is possible to recreate the temperature changes that result in a certain biological effect (such as cell death) during the process of simulated cryosurgery. (C) 2000 Academic Press.",
author = "Wen-Horng Yang and Peng, {Hsin Hsin} and Hsien-Chang Chang and Shih-Yu Shen and Chao-Liang Wu and Chih-Han Chang",
year = "2000",
month = "1",
day = "1",
doi = "10.1006/cryo.2000.2235",
language = "English",
volume = "40",
pages = "159--170",
journal = "Cryobiology",
issn = "0011-2240",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - An in vitro monitoring system for simulated thermal process in cryosurgery

AU - Yang, Wen-Horng

AU - Peng, Hsin Hsin

AU - Chang, Hsien-Chang

AU - Shen, Shih-Yu

AU - Wu, Chao-Liang

AU - Chang, Chih-Han

PY - 2000/1/1

Y1 - 2000/1/1

N2 - This paper describes a new in vitro experimental model that records temperature changes over a culture plate, which then can be used to assess the biological effects of cryosurgery. A cryoprobe and 16 thermocouples set up by a computer control system were used to monitor the temperature changes during freezing and thawing in a culture plate, and the data were used to create a temperature profile of the entire plate. Location of the thermocouples was confirmed by a digital camera viewing from under the plate, and temperature changes at any point in the interpolated areas were estimated using a curve fitting method. The estimated temperature was checked by sampling with four additional randomly placed thermocouples. Linear regression analysis showed that the estimated temperature and measured temperature were very close (correlation coefficients 0.98-0.99). MBT-2 tumor cells were cultured and then subjected to simulated cryosurgery. The surviving cells were stained with crystal violet and the cell death boundary was detected by image processing. Temperature history at the cell death boundary was retrieved and analyzed. With this system it is possible to recreate the temperature changes that result in a certain biological effect (such as cell death) during the process of simulated cryosurgery. (C) 2000 Academic Press.

AB - This paper describes a new in vitro experimental model that records temperature changes over a culture plate, which then can be used to assess the biological effects of cryosurgery. A cryoprobe and 16 thermocouples set up by a computer control system were used to monitor the temperature changes during freezing and thawing in a culture plate, and the data were used to create a temperature profile of the entire plate. Location of the thermocouples was confirmed by a digital camera viewing from under the plate, and temperature changes at any point in the interpolated areas were estimated using a curve fitting method. The estimated temperature was checked by sampling with four additional randomly placed thermocouples. Linear regression analysis showed that the estimated temperature and measured temperature were very close (correlation coefficients 0.98-0.99). MBT-2 tumor cells were cultured and then subjected to simulated cryosurgery. The surviving cells were stained with crystal violet and the cell death boundary was detected by image processing. Temperature history at the cell death boundary was retrieved and analyzed. With this system it is possible to recreate the temperature changes that result in a certain biological effect (such as cell death) during the process of simulated cryosurgery. (C) 2000 Academic Press.

UR - http://www.scopus.com/inward/record.url?scp=0034122745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034122745&partnerID=8YFLogxK

U2 - 10.1006/cryo.2000.2235

DO - 10.1006/cryo.2000.2235

M3 - Article

C2 - 10788315

AN - SCOPUS:0034122745

VL - 40

SP - 159

EP - 170

JO - Cryobiology

JF - Cryobiology

SN - 0011-2240

IS - 2

ER -