Different body segments have different thermal characteristics and sleeping postures change unconsciously during the night. Thus, the movements of the extremities should be taken into consideration in a bedding system design. The design guidelines propose in this study is enhance pressure balance, the physiological balance of microclimate, and improvement of distal skin blood circulation. An innovative mattress design has been developed in this study. The proposed mattress has a five-layer sandwiched structure. Twelve healthy participants had general level in BMI were recruited in 3-day sleep monitoring including one habit adjustment night in simulated laboratory condition to evaluate the original and design mattresses. The consequence indicated mattress design considered thermal comfort efficiently on the basis of position change improved sleep efficient, loss sleep disturbance by loss mean activity and arousal time.