An integrated chip capable of performing sample pretreatment and nucleic acid amplification for HIV-1 detection

Jung Hao Wang, Lie Cheng, Chih Hung Wang, Wei Shuo Ling, Shainn Wei Wang, Gwo Bin Lee

研究成果: Article同行評審

29 引文 斯高帕斯(Scopus)

摘要

This study reports on a microfluidic system equipped with a sample pretreatment device and a nucleic acid amplification device for the rapid diagnosis of the human immunodeficiency virus-1 (HIV-1). The system analyzed proviral deoxyribonucleic acid (DNA) from an HIV-infected Jurkat T cell line. In order to ensure accurate diagnosis among other prevalent B-type strains, simultaneous detections of four conserved HIV-1 B-type DNA fragments were performed in this integrated microfluidic system. The entire protocol including cell lysis, extraction of DNA, polymerase chain reaction (PCR), and optical detection were successfully integrated in order to perform a rapid, automated diagnosis. Experimental results showed that four primer sets with conserved HIV-1 B-type sequences specific for the 167-bp RU5 promoter region, the 424-bp int, the 117-bp tat, and the 162-bp vpr coding regions were successfully amplified from the respective regions of the proviral DNA, even from a single infected cell. This accurate real-time detection was achieved within 95. min using the integrated optical system.

原文English
頁(從 - 到)484-491
頁數8
期刊Biosensors and Bioelectronics
41
發行號1
DOIs
出版狀態Published - 2013 三月 15

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 生物物理學
  • 生物醫學工程
  • 電化學

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