An integrated microfluidic loop-mediated isothermal amplification platform for koi herpesvirus detection

Shih Chu Chen, Chan Chiung Liu, Yao Nan Wang, Lung Ming Fu, Sheng Hong Shih

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)


An integrated microfluidic loop-mediated isothermal amplification (LAMP) platform consisting of a microfluidic chip and a portable operating system is proposed for koi herpesvirus (KHV) detection applications. The microfluidic chip is fabricated using a CO2 laser system and comprises a sample chamber, two reagent chambers, a mixing chamber, a serpentine channel, a reaction chamber, and three flow rectifiers based on PDMS membranes. The main components of the operating system include a power source, a time relay, a microfluidic chip holder, four gas pressure valves, a microheater, and a temperature controller. In the proposed detection method, the KHV sample and LAMP reagent are loaded into the sample and reagent chambers, respectively, and are mixed under the effects of an external gas pressure driving force. The mixed solution is then heated at a temperature of approximately 63 °C for 60 min in order to replicate the KHV sample. Finally, the replicated sample is mixed with SYBR Green I dye in order to facilitate fluorescence-based KHV detection. The feasibility of the proposed microfluidic platform is confirmed by comparing the detection results obtained for a sample containing 10 ng/μL KHV with those obtained using a gel electrophoresis PCR-based method. The experimental results shows that the proposed LAMP platform has a KHV detection limit of around 3 × 10−2 ng/μL. Overall, the results presented in this study show that the proposed microfluidic platform provides a compact, reliable and portable tool for KHV detection purposes.

頁(從 - 到)1828-1834
期刊Chemical Engineering Journal
出版狀態Published - 2018 二月 15

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

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