An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process

Priya Gopinathan, Lien Yu Hung, Chih Hung Wang, Nai Jung Chiang, Yu Chun Wang, Yan-Shen Shan, Gwo Bin Lee

研究成果: Conference contribution

摘要

Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.

原文English
主出版物標題20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
發行者Chemical and Biological Microsystems Society
頁面930-931
頁數2
ISBN(電子)9780979806490
出版狀態Published - 2016 一月 1
事件20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
持續時間: 2016 十月 92016 十月 13

出版系列

名字20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
國家Ireland
城市Dublin
期間16-10-0916-10-13

指紋

Microfluidics
Screening
Biomarkers
Ligands

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

引用此文

Gopinathan, P., Hung, L. Y., Wang, C. H., Chiang, N. J., Wang, Y. C., Shan, Y-S., & Lee, G. B. (2016). An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. 於 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (頁 930-931). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.
Gopinathan, Priya ; Hung, Lien Yu ; Wang, Chih Hung ; Chiang, Nai Jung ; Wang, Yu Chun ; Shan, Yan-Shen ; Lee, Gwo Bin. / An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. 頁 930-931 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).
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title = "An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process",
abstract = "Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.",
author = "Priya Gopinathan and Hung, {Lien Yu} and Wang, {Chih Hung} and Chiang, {Nai Jung} and Wang, {Yu Chun} and Yan-Shen Shan and Lee, {Gwo Bin}",
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Gopinathan, P, Hung, LY, Wang, CH, Chiang, NJ, Wang, YC, Shan, Y-S & Lee, GB 2016, An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. 於 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, 頁 930-931, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16-10-09.

An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. / Gopinathan, Priya; Hung, Lien Yu; Wang, Chih Hung; Chiang, Nai Jung; Wang, Yu Chun; Shan, Yan-Shen; Lee, Gwo Bin.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 930-931 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

研究成果: Conference contribution

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AU - Gopinathan, Priya

AU - Hung, Lien Yu

AU - Wang, Chih Hung

AU - Chiang, Nai Jung

AU - Wang, Yu Chun

AU - Shan, Yan-Shen

AU - Lee, Gwo Bin

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N2 - Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.

AB - Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.

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Gopinathan P, Hung LY, Wang CH, Chiang NJ, Wang YC, Shan Y-S 等. An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process. 於 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 930-931. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).