An integrated microfluidic platform for screening of aptamers specific to cholangiocarcinoma by utilizing on-chip cell selex process

Priya Gopinathan, Lien Yu Hung, Chih Hung Wang, Nai Jung Chiang, Yu Chun Wang, Yan Shen Shan, Gwo Bin Lee

研究成果: Conference contribution

摘要

Cholangiocarcinoma (CCA) is a very aggressive biliary tract malignancy and currently there is no reliable biomarker for diagnosis. In this work, an integrated microfluidic platform capable of performing cell-based systematic evolution of ligands by exponential enrichment (Cell SELEX) process with limited human intervention has been demonstrated for screening of aptamers specific to CCA. Our new magnetic-bead-based microfluidic platform is capable of reducing the time consumed to a 1/6th as compared to previous works. Promising aptamer candidates could be successfully selected in just 5 or 6 cycles as compared to the conventional 15+ cycles.

原文English
主出版物標題20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
發行者Chemical and Biological Microsystems Society
頁面930-931
頁數2
ISBN(電子)9780979806490
出版狀態Published - 2016 一月 1
事件20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
持續時間: 2016 十月 92016 十月 13

出版系列

名字20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
國家Ireland
城市Dublin
期間16-10-0916-10-13

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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