An Integrated Microfluidic Platform to Detect FXYD2 RNA Expression in Ascites for Diagnosis of Ovarian Clear Cell Carcinoma

Yi Da Chung, Yuan Jhe Chuang, Chang Ni Lin, Keng Fu Hsu, Gwo Bin Lee

研究成果: Conference contribution

摘要

An integrated microfluidic platform was developed to automate the entire procedures from cancer cell capturing in ascites, RNA extraction, and on-chip quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for diagnosis of ovarian clear cell carcinoma (OCCC), which is a subtype of ovarian cancer (OvCa) with high mortality. FXYD2 gene expression was found highly associated with OCCC. A new protocol for detecting FXYD2 gene expression by referencing housekeeping gene was realized on the integrated microfluidic system. This detection method can be applied for diagnosis or prognosis monitoring of OCCC based on detection of the target gene expression level in liquid biopsy.

原文English
主出版物標題34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
發行者Institute of Electrical and Electronics Engineers Inc.
頁面989-992
頁數4
ISBN(電子)9781665419123
DOIs
出版狀態Published - 2021 一月 25
事件34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 - Virtual, Gainesville, United States
持續時間: 2021 一月 252021 一月 29

出版系列

名字Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
2021-January
ISSN(列印)1084-6999

Conference

Conference34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
國家/地區United States
城市Virtual, Gainesville
期間21-01-2521-01-29

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 凝聚態物理學
  • 機械工業
  • 電氣與電子工程

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