An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers

Chen Hsun Weng, Lien Yu Hung, Hsin I. Lin, I. Shan Hsieh, Shu Chu Shiesh, Yu Ling Chen, Gwo Bin Lee

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.

原文English
主出版物標題15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
頁面2086-2088
頁數3
3
出版狀態Published - 2011
事件15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
持續時間: 2011 十月 22011 十月 6

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
國家United States
城市Seattle, WA
期間11-10-0211-10-06

    指紋

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

引用此

Weng, C. H., Hung, L. Y., Lin, H. I., Hsieh, I. S., Shiesh, S. C., Chen, Y. L., & Lee, G. B. (2011). An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers. 於 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (卷 3, 頁 2086-2088)