An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers

Chen Hsun Weng; Lien Yu Hung; Hsin I. Lin; I. Shan Hsieh; Shu Chu Shiesh; Yu Ling Chen; Gwo Bin Lee

An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers

Chen Hsun Weng, Lien Yu Hung, Hsin I. Lin, I. Shan Hsieh, Shu Chu Shiesh, Yu Ling Chen, Gwo Bin Lee

研究成果: Conference contribution

摘要

A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.

原文	English
主出版物標題	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
頁面	2086-2088
頁數	3
卷	3
出版狀態	Published - 2011
事件	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States 持續時間: 2011 10月 2 → 2011 10月 6

Other

Other	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
國家/地區	United States
城市	Seattle, WA
期間	11-10-02 → 11-10-06

All Science Journal Classification (ASJC) codes

控制與系統工程

UN SDG

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其它文件與鏈接

引用此

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title = "An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers",

abstract = "A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.",

author = "Weng, {Chen Hsun} and Hung, {Lien Yu} and Lin, {Hsin I.} and Hsieh, {I. Shan} and Shiesh, {Shu Chu} and Chen, {Yu Ling} and Lee, {Gwo Bin}",

year = "2011",

language = "English",

isbn = "9781618395955",

volume = "3",

pages = "2086--2088",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",

}

Weng, CH, Hung, LY, Lin, HI, Hsieh, IS, Shiesh, SC, Chen, YL & Lee, GB 2011, An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers. 於 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 卷 3, 頁 2086-2088, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11-10-02.

TY - GEN

T1 - An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers

AU - Weng, Chen Hsun

AU - Hung, Lien Yu

AU - Lin, Hsin I.

AU - Hsieh, I. Shan

AU - Shiesh, Shu Chu

AU - Chen, Yu Ling

AU - Lee, Gwo Bin

PY - 2011

Y1 - 2011

N2 - A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.

AB - A new microfluidic system involving a progressive selection of highly specific ligands by repeated rounds of partition and amplification from a large combinatorial nucleic acid library to screen tumor cell-specific aptamers was developed in this study. The systematic evolution of ligands by exponential enrichment (SELEX) process can be then automated. With this approach, an aptamer specific to lung cancer cells has been successfully screened. The entire process can be decreased from 2 weeks to only 3 days. The developed Cell-SELEX microsystem can be promising for fast screening of aptamers specific for tumor cells, which can be promising for early diagnosis of cancers and target therapy.

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UR - http://www.scopus.com/inward/citedby.url?scp=84874423281&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84874423281

SN - 9781618395955

VL - 3

SP - 2086

EP - 2088

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

An integrated microfluidic system for automating on-chip selex process to screen tumor cell-specific aptamers

摘要

Other

All Science Journal Classification (ASJC) codes

UN SDG

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