An integrated microfluidic system for rapid screening of alpha-fetoprotein aptamers

Chao June Huang, Hsin I. Lin, Shu-Chu Shiesh, Gwo Bin Lee

研究成果: Conference contribution

摘要

Systematic evolution of ligands by exponential enrichment (SELEX) is a screening technique that involves a progressive selection of highly specific ligands by performing repeated rounds of partition and amplification from a large combinatorial nucleic acid library. The products of the selection are usually called aptamers, which are short single-stranded deoxyribonucleic acid (ssDNA) or ribonucleic acid (ssRNA) molecules binding with high affinity to a large variety of target analytes. However, SELEX is an iterative process requiring multiple rounds of extraction and polymerase chain reaction (PCR) amplification that demands significant sampling and process time. In order to address these problems, the study presents a new integrated microfluidic system consisting of an automatic SELEX chip and a competitive assay chip to enhance the aptamer screening efficiency. With this approach, an aptamer specific to alpha-fetoprotein (AFP), which is a biomarker for liver cancer, has been successfully screened.

原文English
主出版物標題2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
頁面185-188
頁數4
DOIs
出版狀態Published - 2011 四月 13
事件24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 - Cancun, Mexico
持續時間: 2011 一月 232011 一月 27

出版系列

名字Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(列印)1084-6999

Other

Other24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
國家Mexico
城市Cancun
期間11-01-2311-01-27

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

指紋 深入研究「An integrated microfluidic system for rapid screening of alpha-fetoprotein aptamers」主題。共同形成了獨特的指紋。

引用此