An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology

Ching Wen Yu; Lien Yu Hung; Chien Yu Fu; Chih Hung Wang; Nai Jung Chiang; Yu Chun Wang; Yan Shen Shan; Gwo Bin Lee

doi:10.1109/NEMS.2016.7758314

An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology

Ching Wen Yu, Lien Yu Hung, Chien Yu Fu, Chih Hung Wang, Nai Jung Chiang, Yu Chun Wang, Yan Shen Shan, Gwo Bin Lee

臨床醫學研究所

研究成果: Conference contribution

1 引文斯高帕斯（Scopus）

摘要

Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.

原文	English
主出版物標題	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
發行者	Institute of Electrical and Electronics Engineers Inc.
頁面	561-564
頁數	4
ISBN（電子）	9781509019472
DOIs	https://doi.org/10.1109/NEMS.2016.7758314
出版狀態	Published - 2016 十一月 28
事件	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, Japan 持續時間: 2016 四月 17 → 2016 四月 20

出版系列

名字	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

Other

Other	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
國家	Japan
城市	Sendai
期間	16-04-17 → 16-04-20

All Science Journal Classification (ASJC) codes

Industrial and Manufacturing Engineering
Mechanical Engineering
Mechanics of Materials
Electronic, Optical and Magnetic Materials

存取文件

10.1109/NEMS.2016.7758314

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Yu, C. W., Hung, L. Y., Fu, C. Y., Wang, C. H., Chiang, N. J., Wang, Y. C., Shan, Y. S., & Lee, G. B. (2016). An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. 於 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 (頁 561-564). [7758314] (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2016.7758314

Yu, Ching Wen ; Hung, Lien Yu ; Fu, Chien Yu ; Wang, Chih Hung ; Chiang, Nai Jung ; Wang, Yu Chun ; Shan, Yan Shen ; Lee, Gwo Bin. / An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. 頁 561-564 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

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title = "An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology",

abstract = "Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.",

author = "Yu, {Ching Wen} and Hung, {Lien Yu} and Fu, {Chien Yu} and Wang, {Chih Hung} and Chiang, {Nai Jung} and Wang, {Yu Chun} and Shan, {Yan Shen} and Lee, {Gwo Bin}",

note = "Funding Information: The authors would like to thank financial supports from Ministry of Science and Technology, Taiwan (104- 2119-M-007-009, 104-2811-E-007-031) and {"}Towards a World-Class University{"} Project (104N2751E1). Partial financial support from NHRI, Taiwan (NHRI-EX104- 10428EI) is greatly appreciated. Publisher Copyright: {\textcopyright} 2016 IEEE.; 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 ; Conference date: 17-04-2016 Through 20-04-2016",

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doi = "10.1109/NEMS.2016.7758314",

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Yu, CW, Hung, LY, Fu, CY, Wang, CH, Chiang, NJ, Wang, YC, Shan, YS & Lee, GB 2016, An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. 於 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016., 7758314, 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Institute of Electrical and Electronics Engineers Inc., 頁 561-564, 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Sendai, Japan, 16-04-17. https://doi.org/10.1109/NEMS.2016.7758314

An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. / Yu, Ching Wen; Hung, Lien Yu; Fu, Chien Yu; Wang, Chih Hung; Chiang, Nai Jung; Wang, Yu Chun; Shan, Yan Shen; Lee, Gwo Bin.

2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 561-564 7758314 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

研究成果: Conference contribution

TY - GEN

T1 - An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology

AU - Yu, Ching Wen

AU - Hung, Lien Yu

AU - Fu, Chien Yu

AU - Wang, Chih Hung

AU - Chiang, Nai Jung

AU - Wang, Yu Chun

AU - Shan, Yan Shen

AU - Lee, Gwo Bin

N1 - Funding Information: The authors would like to thank financial supports from Ministry of Science and Technology, Taiwan (104- 2119-M-007-009, 104-2811-E-007-031) and "Towards a World-Class University" Project (104N2751E1). Partial financial support from NHRI, Taiwan (NHRI-EX104- 10428EI) is greatly appreciated. Publisher Copyright: © 2016 IEEE.

PY - 2016/11/28

Y1 - 2016/11/28

N2 - Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.

AB - Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.

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U2 - 10.1109/NEMS.2016.7758314

DO - 10.1109/NEMS.2016.7758314

M3 - Conference contribution

AN - SCOPUS:85007256358

T3 - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

SP - 561

EP - 564

BT - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

Y2 - 17 April 2016 through 20 April 2016

ER -

Yu CW, Hung LY, Fu CY, Wang CH, Chiang NJ, Wang YC 等. An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. 於 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 561-564. 7758314. (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). https://doi.org/10.1109/NEMS.2016.7758314