摘要
Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.
| 原文 | English |
|---|---|
| 主出版物標題 | 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 |
| 發行者 | Institute of Electrical and Electronics Engineers Inc. |
| 頁面 | 561-564 |
| 頁數 | 4 |
| ISBN（電子） | 9781509019472 |
| DOIs | |
| 出版狀態 | Published - 2016 十一月 28 |
| 事件 | 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, Japan 持續時間: 2016 四月 17 → 2016 四月 20 |
出版系列
| 名字 | 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 |
|---|
Other
| Other | 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 |
|---|---|
| 國家 | Japan |
| 城市 | Sendai |
| 期間 | 16-04-17 → 16-04-20 |
指紋
All Science Journal Classification (ASJC) codes
- Industrial and Manufacturing Engineering
- Mechanical Engineering
- Mechanics of Materials
- Electronic, Optical and Magnetic Materials
引用此文
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An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology. / Yu, Ching Wen; Hung, Lien Yu; Fu, Chien Yu; Wang, Chih Hung; Chiang, Nai Jung; Wang, Yu Chun; Shan, Yan-Shen; Lee, Gwo Bin.
2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 561-564 7758314 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).研究成果: Conference contribution
TY - GEN
T1 - An integrated microfluidic system for screening of peptides specific to cholangiocarcinoma (CCA) cancer cell lines using the phage display technology
AU - Yu, Ching Wen
AU - Hung, Lien Yu
AU - Fu, Chien Yu
AU - Wang, Chih Hung
AU - Chiang, Nai Jung
AU - Wang, Yu Chun
AU - Shan, Yan-Shen
AU - Lee, Gwo Bin
PY - 2016/11/28
Y1 - 2016/11/28
N2 - Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.
AB - Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study, an integrated microfluidic system was proposed to perform on-chip phage display for selection of specific peptides that could identify CCA cell lines. The results showed that we could perform one panning round within 6 hours and the whole processes could be automated. This microfluidic system may be a powerful tool for screening of affinity agents in the clinical diagnosis and target therapeutics for CCA.
UR - http://www.scopus.com/inward/record.url?scp=85007256358&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007256358&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2016.7758314
DO - 10.1109/NEMS.2016.7758314
M3 - Conference contribution
AN - SCOPUS:85007256358
T3 - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
SP - 561
EP - 564
BT - 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
PB - Institute of Electrical and Electronics Engineers Inc.
ER -