An integrated microfluidic system with field-effect-transistor sensor arrays for detecting multiple cardiovascular biomarkers from clinical samples

Anirban Sinha, Tse Yu Tai, Kuang Hsien Li, Priya Gopinathan, Yi Da Chung, Indu Sarangadharan, Hsi Pin Ma, Po Chiun Huang, Shu Chu Shiesh, Yu Lin Wang, Gwo Bin Lee

研究成果: Article同行評審

75 引文 斯高帕斯(Scopus)

摘要

Certain blood-borne biomarkers offer a potent methodology for understanding the risk of cardiovascular diseases (CVDs) with clinicians generally advocating the use of multiple biomarkers for proper risk assessment of CVDs. Herein four such CVDs biomarkers- C-reactive protein (CRP), N-terminal pro b-type natriuretic peptide (NT-proBNP), cardiac troponin I (cTnI), and fibrinogen- were rapidly (5 min) analyzed from clinical samples (~ 4 µL) on an integrated microfluidic platform equipped with 1) immobilized highly specific aptamer probes and 2) field-effect transistor (FET)-based sensor arrays. The calibration curve from the FET sensor arrays showed good agreement in the physiological concentration ranges for CRP (0.1–50 mg/L), NT-proBNP (50–10,000 pg/mL), cTnI (1–10,000 pg/mL), and fibrinogen (0.1–5 mg/mL). The developed prototype of this fully automated portable device requires minimal reagent and sample inputs and consequently shows great promise for next-generation point-of-care devices assaying multiple CVDs biomarkers in clinical samples.

原文English
頁(從 - 到)155-163
頁數9
期刊Biosensors and Bioelectronics
129
DOIs
出版狀態Published - 2019 3月 15

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 生物物理學
  • 生物醫學工程
  • 電化學

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