An Integrated, Multiplex Digital PCR-Based Microfluidic System for Quantification of Two Microrna Biomarkers for Diagnosis of Ovarian Cancer

Chi Chien Huang, Chia Yu Sung, Yi Sin Chen, Keng Fu Hsu, Gwo Bin Lee

研究成果: Conference contribution

摘要

Extracellular vesicle (EV)-derived microRNA (miRNA) has been reported to be a novel biomarker for diagnosis of ovarian cancer (OvCa). This study reported an integrated microfluidic system to automate extraction and quantification of EV-miRNA on a single chip within 120 min. The EV capture rate was 1.8-fold higher than our previous work (45%) due to the use of anti-CD63 beads and anti-EpCAM beads. Moreover, two miRNA biomarkers (i.e. miR-21 and miR-200a) were ultaneously detected and quantified with multiplex PCR while the resulting calibration curves exhibited a high linearity without false-positive signals, which could be carried out on a digital PCR module. With this approach, a precise and specific diagnostic tool for OvCa could be feasible.

原文English
主出版物標題35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
發行者Institute of Electrical and Electronics Engineers Inc.
頁面91-94
頁數4
ISBN(電子)9781665409117
DOIs
出版狀態Published - 2022
事件35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 - Tokyo, Japan
持續時間: 2022 1月 92022 1月 13

出版系列

名字Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
2022-January
ISSN(列印)1084-6999

Conference

Conference35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
國家/地區Japan
城市Tokyo
期間22-01-0922-01-13

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 凝聚態物理學
  • 機械工業
  • 電氣與電子工程

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