An intergated microfluidic system for detecting human immunodeficiency virus in blood samples

Wen Hsin Chang, Jung Hao Wang, Wei Shuo Ling, Lie Cheng, Chih Hung Wang, Shainn-Wei Wang, Gwo Bin Lee

研究成果: Conference contribution

摘要

To date, 25 million deaths have been caused by human immunodeficiency virus (HIV) infections. However, the lack of effective vaccines and drugs still leads to a high transmission rate of HIV. It was estimated that 2.7 million new infections occurred in 2011. Early and accurate diagnosis is essential to control the HIV transmission. Thus, many efforts have been made to explore a rapid and accurate method for HIV detection. The capabilities of antibody-based determination and nucleic-acid-based molecular diagnosis to detect HIV infections have been verified. Yet these methods have some intrinsic drawbacks. For example, antibody-based assays involve a lengthy process and may have an open-window issue while polymerase chain reaction (PCR)-based assays require delicate equipment and well-trained personnel. In this work, we present an integrated microfluidic system using a rolling circle amplification (RCA) assay which could automatically detect DNA fragment of HIV-1. With this approach, the HIV-1 could be detected automatically to reduce the risk of contamination with no human intervention. Additionally, the specially designed hook primer coated on the magnetic beads and a padlock probe both contribute to RNA extraction with higher specificity such that this developed system is capable of detecting HIV-1 from blood cells directly with improved accuracy. Successful demonstration for detection of HIV-1 DNA fragment has been achieved by using the proposed microfluidic platform.

原文English
主出版物標題IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
頁面903-906
頁數4
DOIs
出版狀態Published - 2013 四月 2
事件IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan
持續時間: 2013 一月 202013 一月 24

出版系列

名字Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(列印)1084-6999

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
國家Taiwan
城市Taipei
期間13-01-2013-01-24

指紋

human immunodeficiency virus
Viruses
Microfluidics
blood
Blood
infectious diseases
Assays
antibodies
Antibodies
DNA
deoxyribonucleic acid
fragments
vaccines
polymerase chain reaction
primers
blood cells
Vaccines
hooks
Hooks
Polymerase chain reaction

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

引用此文

Chang, W. H., Wang, J. H., Ling, W. S., Cheng, L., Wang, C. H., Wang, S-W., & Lee, G. B. (2013). An intergated microfluidic system for detecting human immunodeficiency virus in blood samples. 於 IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (頁 903-906). [6474390] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474390
Chang, Wen Hsin ; Wang, Jung Hao ; Ling, Wei Shuo ; Cheng, Lie ; Wang, Chih Hung ; Wang, Shainn-Wei ; Lee, Gwo Bin. / An intergated microfluidic system for detecting human immunodeficiency virus in blood samples. IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. 頁 903-906 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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title = "An intergated microfluidic system for detecting human immunodeficiency virus in blood samples",
abstract = "To date, 25 million deaths have been caused by human immunodeficiency virus (HIV) infections. However, the lack of effective vaccines and drugs still leads to a high transmission rate of HIV. It was estimated that 2.7 million new infections occurred in 2011. Early and accurate diagnosis is essential to control the HIV transmission. Thus, many efforts have been made to explore a rapid and accurate method for HIV detection. The capabilities of antibody-based determination and nucleic-acid-based molecular diagnosis to detect HIV infections have been verified. Yet these methods have some intrinsic drawbacks. For example, antibody-based assays involve a lengthy process and may have an open-window issue while polymerase chain reaction (PCR)-based assays require delicate equipment and well-trained personnel. In this work, we present an integrated microfluidic system using a rolling circle amplification (RCA) assay which could automatically detect DNA fragment of HIV-1. With this approach, the HIV-1 could be detected automatically to reduce the risk of contamination with no human intervention. Additionally, the specially designed hook primer coated on the magnetic beads and a padlock probe both contribute to RNA extraction with higher specificity such that this developed system is capable of detecting HIV-1 from blood cells directly with improved accuracy. Successful demonstration for detection of HIV-1 DNA fragment has been achieved by using the proposed microfluidic platform.",
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Chang, WH, Wang, JH, Ling, WS, Cheng, L, Wang, CH, Wang, S-W & Lee, GB 2013, An intergated microfluidic system for detecting human immunodeficiency virus in blood samples. 於 IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013., 6474390, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 頁 903-906, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, 13-01-20. https://doi.org/10.1109/MEMSYS.2013.6474390

An intergated microfluidic system for detecting human immunodeficiency virus in blood samples. / Chang, Wen Hsin; Wang, Jung Hao; Ling, Wei Shuo; Cheng, Lie; Wang, Chih Hung; Wang, Shainn-Wei; Lee, Gwo Bin.

IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 903-906 6474390 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

研究成果: Conference contribution

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AU - Lee, Gwo Bin

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AB - To date, 25 million deaths have been caused by human immunodeficiency virus (HIV) infections. However, the lack of effective vaccines and drugs still leads to a high transmission rate of HIV. It was estimated that 2.7 million new infections occurred in 2011. Early and accurate diagnosis is essential to control the HIV transmission. Thus, many efforts have been made to explore a rapid and accurate method for HIV detection. The capabilities of antibody-based determination and nucleic-acid-based molecular diagnosis to detect HIV infections have been verified. Yet these methods have some intrinsic drawbacks. For example, antibody-based assays involve a lengthy process and may have an open-window issue while polymerase chain reaction (PCR)-based assays require delicate equipment and well-trained personnel. In this work, we present an integrated microfluidic system using a rolling circle amplification (RCA) assay which could automatically detect DNA fragment of HIV-1. With this approach, the HIV-1 could be detected automatically to reduce the risk of contamination with no human intervention. Additionally, the specially designed hook primer coated on the magnetic beads and a padlock probe both contribute to RNA extraction with higher specificity such that this developed system is capable of detecting HIV-1 from blood cells directly with improved accuracy. Successful demonstration for detection of HIV-1 DNA fragment has been achieved by using the proposed microfluidic platform.

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Chang WH, Wang JH, Ling WS, Cheng L, Wang CH, Wang S-W 等. An intergated microfluidic system for detecting human immunodeficiency virus in blood samples. 於 IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 903-906. 6474390. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474390