An intermediate concentration of calcium with antioxidant supplement in culture medium enhances proliferation and decreases the aging of bone marrow mesenchymal stem cells

Chung Da Yang, Shu Chun Chuang, Tsung Lin Cheng, Mon Juan Lee, Hui Ting Chen, Sung Yen Lin, Hsuan Ti Huang, Cheng Jung Ho, Yi Shan Lin, Lin Kang, Mei Ling Ho, Je Ken Chang, Chung Hwan Chen

研究成果: Article同行評審

摘要

Human bone marrow stem cells (HBMSCs) are isolated from the bone marrow. Stem cells can self-renew and differentiate into various types of cells. They are able to regenerate kinds of tissue that are potentially used for tissue engineering. To maintain and expand these cells under culture conditions is difficult—they are easily triggered for differentiation or death. In this study, we describe a new culture formula to culture isolated HBMSCs. This new formula was modified from NCDB 153, a medium with low calcium, supplied with 5% FBS, extra growth factor added to it, and supplemented with N-acetyl-L-cysteine and L-ascorbic acid-2-phosphate to maintain the cells in a steady stage. The cells retain these characteristics as primarily isolated HBMSCs. Moreover, our new formula keeps HBMSCs with high proliferation rate and multiple linage differentiation ability, such as osteoblastogenesis, chondrogenesis, and adipogenesis. It also retains HBMSCs with stable chromosome, DNA, telomere length, and telomerase activity, even after long-term culture. Senescence can be minimized under this new formulation and carcinogenesis of stem cells can also be prevented. These modifications greatly enhance the survival rate, growth rate, and basal characteristics of isolated HBMSCs, which will be very helpful in stem cell research.

原文English
文章編號2095
頁(從 - 到)1-18
頁數18
期刊International journal of molecular sciences
22
發行號4
DOIs
出版狀態Published - 2021 二月 2

All Science Journal Classification (ASJC) codes

  • 催化
  • 分子生物學
  • 光譜
  • 電腦科學應用
  • 物理與理論化學
  • 有機化學
  • 無機化學

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