An optical diagnostic system using isothermal amplification technique for Phalaenopsis orchids

Wen Hsin Chang, Sung Yi Yang, Chih Hung Wang, Ping Chen Li, Fuh Jyh Jan, Tzong-Yueh Chen, Gwo Bin Lee

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

Early detection of pathogens is important for surveillance and control of infectious diseases among Phalaenopsis orchids. Therefore, the current study presents an integrated microfluidic system for rapid and automatic detection of Phalaenopsis orchid viruses. The entire process, including pathogen-specific ribonucleic acid (RNA) purification, nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection by measuring turbidity change, can be automatically performed on a single chip within 65 minutes. This is the first time that an integrated microfluidic system for the detection of Phalaenopsis orchid viruses has been demonstrated. The specificity and sensitivity of the system were also explored in this study to validate its performance. One of the most prevalent orchid viruses, Cymbidium mosaic virus (CymMV), was used in current study to demonstrate the capabilities of the developed system.

原文English
主出版物標題2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
頁面204-207
頁數4
DOIs
出版狀態Published - 2012 六月 1
事件7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 - Kyoto, Japan
持續時間: 2012 三月 52012 三月 8

出版系列

名字2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Other

Other7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
國家Japan
城市Kyoto
期間12-03-0512-03-08

All Science Journal Classification (ASJC) codes

  • Engineering (miscellaneous)

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