An optical-induced platform for multiple genes transfection

Hsin Tzu Kuo, You Hsun Lee, Chih Hung Wang, Chen-Min Chang, Gwo Bin Lee

研究成果: Conference contribution

3 引文 (Scopus)

摘要

Gene transfection is an important technology in biological applications. Electroporation is one of gene transfection methods that deliver extracellular genetic materials into cells by using a high electrical field to forming pores on cell surface. However, the high voltage provided by an electroporator may impair cells to survive and cause low efficiency of yield rate. In this study, a new platform was developed for gene transfection under a lower applied voltage by utilizing an optical induced non-uniform electric field. To obtain higher transfection efficiency, a multi-spot optical image was projected to the gene transfection chip, resulting in localized non-uniform electric fields generated from these optical patterns. The experimental results in this study showed that one or more plasmid carried fluorescence genes could be effectively transfected into mammalian cells and the cells successfully expressed foreign proteins by using the developed optical-induced gene transfection platform. Moreover, the transfection efficiency of optical-induced gene transfection platform was significantly higher than the traditional electroporation technology. This developed platform may provide a simpler and more efficient tool for gene transfection.

原文English
主出版物標題Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
發行者Chemical and Biological Microsystems Society
頁面1657-1659
頁數3
ISBN(列印)9780979806452
出版狀態Published - 2012 一月 1
事件16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
持續時間: 2012 十月 282012 十一月 1

出版系列

名字Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
國家Japan
城市Okinawa
期間12-10-2812-11-01

指紋

Genes
Electric fields
Electric potential
Plasmids
Fluorescence
Cells
Proteins

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

引用此文

Kuo, H. T., Lee, Y. H., Wang, C. H., Chang, C-M., & Lee, G. B. (2012). An optical-induced platform for multiple genes transfection. 於 Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (頁 1657-1659). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.
Kuo, Hsin Tzu ; Lee, You Hsun ; Wang, Chih Hung ; Chang, Chen-Min ; Lee, Gwo Bin. / An optical-induced platform for multiple genes transfection. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. 頁 1657-1659 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
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Kuo, HT, Lee, YH, Wang, CH, Chang, C-M & Lee, GB 2012, An optical-induced platform for multiple genes transfection. 於 Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, 頁 1657-1659, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12-10-28.

An optical-induced platform for multiple genes transfection. / Kuo, Hsin Tzu; Lee, You Hsun; Wang, Chih Hung; Chang, Chen-Min; Lee, Gwo Bin.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1657-1659 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

研究成果: Conference contribution

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AB - Gene transfection is an important technology in biological applications. Electroporation is one of gene transfection methods that deliver extracellular genetic materials into cells by using a high electrical field to forming pores on cell surface. However, the high voltage provided by an electroporator may impair cells to survive and cause low efficiency of yield rate. In this study, a new platform was developed for gene transfection under a lower applied voltage by utilizing an optical induced non-uniform electric field. To obtain higher transfection efficiency, a multi-spot optical image was projected to the gene transfection chip, resulting in localized non-uniform electric fields generated from these optical patterns. The experimental results in this study showed that one or more plasmid carried fluorescence genes could be effectively transfected into mammalian cells and the cells successfully expressed foreign proteins by using the developed optical-induced gene transfection platform. Moreover, the transfection efficiency of optical-induced gene transfection platform was significantly higher than the traditional electroporation technology. This developed platform may provide a simpler and more efficient tool for gene transfection.

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Kuo HT, Lee YH, Wang CH, Chang C-M, Lee GB. An optical-induced platform for multiple genes transfection. 於 Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1657-1659. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).