摘要
Gene transfection is an important technology in biological applications. Electroporation is one of gene transfection methods that deliver extracellular genetic materials into cells by using a high electrical field to forming pores on cell surface. However, the high voltage provided by an electroporator may impair cells to survive and cause low efficiency of yield rate. In this study, a new platform was developed for gene transfection under a lower applied voltage by utilizing an optical induced non-uniform electric field. To obtain higher transfection efficiency, a multi-spot optical image was projected to the gene transfection chip, resulting in localized non-uniform electric fields generated from these optical patterns. The experimental results in this study showed that one or more plasmid carried fluorescence genes could be effectively transfected into mammalian cells and the cells successfully expressed foreign proteins by using the developed optical-induced gene transfection platform. Moreover, the transfection efficiency of optical-induced gene transfection platform was significantly higher than the traditional electroporation technology. This developed platform may provide a simpler and more efficient tool for gene transfection.
| 原文 | English |
|---|---|
| 主出版物標題 | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
| 發行者 | Chemical and Biological Microsystems Society |
| 頁面 | 1657-1659 |
| 頁數 | 3 |
| ISBN（列印） | 9780979806452 |
| 出版狀態 | Published - 2012 一月 1 |
| 事件 | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan 持續時間: 2012 十月 28 → 2012 十一月 1 |
出版系列
| 名字 | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|
Other
| Other | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|---|
| 國家 | Japan |
| 城市 | Okinawa |
| 期間 | 12-10-28 → 12-11-01 |
指紋
All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Bioengineering
引用此文
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An optical-induced platform for multiple genes transfection. / Kuo, Hsin Tzu; Lee, You Hsun; Wang, Chih Hung; Chang, Chen-Min; Lee, Gwo Bin.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1657-1659 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).研究成果: Conference contribution
TY - GEN
T1 - An optical-induced platform for multiple genes transfection
AU - Kuo, Hsin Tzu
AU - Lee, You Hsun
AU - Wang, Chih Hung
AU - Chang, Chen-Min
AU - Lee, Gwo Bin
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Gene transfection is an important technology in biological applications. Electroporation is one of gene transfection methods that deliver extracellular genetic materials into cells by using a high electrical field to forming pores on cell surface. However, the high voltage provided by an electroporator may impair cells to survive and cause low efficiency of yield rate. In this study, a new platform was developed for gene transfection under a lower applied voltage by utilizing an optical induced non-uniform electric field. To obtain higher transfection efficiency, a multi-spot optical image was projected to the gene transfection chip, resulting in localized non-uniform electric fields generated from these optical patterns. The experimental results in this study showed that one or more plasmid carried fluorescence genes could be effectively transfected into mammalian cells and the cells successfully expressed foreign proteins by using the developed optical-induced gene transfection platform. Moreover, the transfection efficiency of optical-induced gene transfection platform was significantly higher than the traditional electroporation technology. This developed platform may provide a simpler and more efficient tool for gene transfection.
AB - Gene transfection is an important technology in biological applications. Electroporation is one of gene transfection methods that deliver extracellular genetic materials into cells by using a high electrical field to forming pores on cell surface. However, the high voltage provided by an electroporator may impair cells to survive and cause low efficiency of yield rate. In this study, a new platform was developed for gene transfection under a lower applied voltage by utilizing an optical induced non-uniform electric field. To obtain higher transfection efficiency, a multi-spot optical image was projected to the gene transfection chip, resulting in localized non-uniform electric fields generated from these optical patterns. The experimental results in this study showed that one or more plasmid carried fluorescence genes could be effectively transfected into mammalian cells and the cells successfully expressed foreign proteins by using the developed optical-induced gene transfection platform. Moreover, the transfection efficiency of optical-induced gene transfection platform was significantly higher than the traditional electroporation technology. This developed platform may provide a simpler and more efficient tool for gene transfection.
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UR - http://www.scopus.com/inward/citedby.url?scp=84891413312&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84891413312
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1657
EP - 1659
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
ER -