TY - GEN
T1 - An optical measurement using the DNA hybridization method for specific species of bacteria detection
AU - Yeh, Chia Hsien
AU - Chang, Yu Huai
AU - Lin, Hong Ping
AU - Chang, Tsung Chain
AU - Lin, Yu Cheng
PY - 2010
Y1 - 2010
N2 - This paper reports a developed DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to detect the hybridization signal. There was a significant difference in grayscale between the experimental samples and the negative control after 18 min. The detection limit for a DNA sample is 8.25 ng/mL with 5 μM of probe and 10-3 μM of AuNPs-streptavidin. The probe specific tests for different DNA samples were discussed: (1) the probe specific tests for various species of bacteria, and (2) the probe specific tests between the various strains of a single species and various species of a single genus. The developed DNA hybridization method is easy, convenient, and rapid.
AB - This paper reports a developed DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to detect the hybridization signal. There was a significant difference in grayscale between the experimental samples and the negative control after 18 min. The detection limit for a DNA sample is 8.25 ng/mL with 5 μM of probe and 10-3 μM of AuNPs-streptavidin. The probe specific tests for different DNA samples were discussed: (1) the probe specific tests for various species of bacteria, and (2) the probe specific tests between the various strains of a single species and various species of a single genus. The developed DNA hybridization method is easy, convenient, and rapid.
UR - http://www.scopus.com/inward/record.url?scp=79951940165&partnerID=8YFLogxK
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U2 - 10.1109/ICSENS.2010.5690160
DO - 10.1109/ICSENS.2010.5690160
M3 - Conference contribution
AN - SCOPUS:79951940165
SN - 9781424481682
T3 - Proceedings of IEEE Sensors
SP - 474
EP - 476
BT - IEEE Sensors 2010 Conference, SENSORS 2010
T2 - 9th IEEE Sensors Conference 2010, SENSORS 2010
Y2 - 1 November 2010 through 4 November 2010
ER -