An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection

Yi-Min Chen, Ching Hung Shih, Hsiao Chien Liu, Cheng Lung Wu, Chin Chiu Lin, Han-Ching Wang, Tzong-Yueh Chen, Huey Lang Yang, John Han You Lin

研究成果: Article

15 引文 (Scopus)

摘要

Oral vaccination is the most desirable immunization method by which to prevent fish disease occurring at the early larval stage. We developed an Escherichia coli-based oral nervous necrosis virus (NNV) subunit vaccine and demonstrated its effectiveness in orange spotted grouper (Epinephelus coioides) fry. However, the incubation period was determined to be over 2. weeks. To enhance the efficacy and shorten the incubation period, a safe and digestible biological adjuvant should be found. Vibrio anguillarum, a common marine bacterium with immune-stimulatory capability was selected as an expression host. An expression vector containing its heat shock protein 60 gene (groE) as an inducible promoter was constructed to express recombinant NNV coat protein as an antigen. The recombinant V. anguillarum was inactivated and encapsulated in Artemia nauplii to formulate a V. anguillarum-based oral vaccine. Compared to the E. coli-based oral vaccine, this novel oral vaccine not only elicited a higher expression of Mx (encoding for an anti-viral protein) in the brain and viscera but also a higher specific antibody titer against NNV in the first week after inoculation. Meanwhile, a higher survival rate compared to an equal dosage of an E. coli-based oral vaccine was also noticed in two individual challenge trials, indicating that an antigen expressed in an V. anguillarum-based oral vaccine could enhance efficacy in a shorter incubation period and could reduce the risk of NNV infection.

原文English
頁(從 - 到)26-33
頁數8
期刊Aquaculture
321
發行號1-2
DOIs
出版狀態Published - 2011 十一月 16

指紋

Vibrio anguillarum
vaccine
mouth
virus
necrosis
vaccines
viruses
Epinephelus coioides
incubation
Escherichia coli
antigen
protein
antiviral proteins
antigens
oral vaccination
fish diseases
immunization
vaccination
subunit vaccines
heat shock

All Science Journal Classification (ASJC) codes

  • Aquatic Science

引用此文

Chen, Yi-Min ; Shih, Ching Hung ; Liu, Hsiao Chien ; Wu, Cheng Lung ; Lin, Chin Chiu ; Wang, Han-Ching ; Chen, Tzong-Yueh ; Yang, Huey Lang ; Lin, John Han You. / An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection. 於: Aquaculture. 2011 ; 卷 321, 編號 1-2. 頁 26-33.
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abstract = "Oral vaccination is the most desirable immunization method by which to prevent fish disease occurring at the early larval stage. We developed an Escherichia coli-based oral nervous necrosis virus (NNV) subunit vaccine and demonstrated its effectiveness in orange spotted grouper (Epinephelus coioides) fry. However, the incubation period was determined to be over 2. weeks. To enhance the efficacy and shorten the incubation period, a safe and digestible biological adjuvant should be found. Vibrio anguillarum, a common marine bacterium with immune-stimulatory capability was selected as an expression host. An expression vector containing its heat shock protein 60 gene (groE) as an inducible promoter was constructed to express recombinant NNV coat protein as an antigen. The recombinant V. anguillarum was inactivated and encapsulated in Artemia nauplii to formulate a V. anguillarum-based oral vaccine. Compared to the E. coli-based oral vaccine, this novel oral vaccine not only elicited a higher expression of Mx (encoding for an anti-viral protein) in the brain and viscera but also a higher specific antibody titer against NNV in the first week after inoculation. Meanwhile, a higher survival rate compared to an equal dosage of an E. coli-based oral vaccine was also noticed in two individual challenge trials, indicating that an antigen expressed in an V. anguillarum-based oral vaccine could enhance efficacy in a shorter incubation period and could reduce the risk of NNV infection.",
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An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection. / Chen, Yi-Min; Shih, Ching Hung; Liu, Hsiao Chien; Wu, Cheng Lung; Lin, Chin Chiu; Wang, Han-Ching; Chen, Tzong-Yueh; Yang, Huey Lang; Lin, John Han You.

於: Aquaculture, 卷 321, 編號 1-2, 16.11.2011, p. 26-33.

研究成果: Article

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