An ovariectomy-induced rabbit osteoporotic model: A new perspective

Nathan Robert Wanderman, Cindy Mallet, Hugo Giambini, Nirong Bao, Chunfeng Zhao, Kai Nan An, Brett A. Freedman, Ahmad Nassr

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Study Design: Experimental Animal Model. Purpose: The aim of our study was to validate a pure bilateral ovariectomy (OVX) female New Zealand white rabbit model of postmenopausal osteoporosis utilizing animal-sparing in vivo techniques for evaluating bone mineral density (BMD). We also sought to demonstrate that bilateral OVX in female New Zealand white rabbits can produce diminished BMD in the spinal column and simulate osteoporosis, without the need for adjuvant chemotherapeutic agents (i.e., no additional glucocorticosteroids or other drugs were used for stimulating accelerated BMD loss), which can be assessed by in vivo BMD testing. Overview of Literature: Multiple animal models of postmenopausal osteoporosis have been described. Rat ovariectomy models have been successful, but are limited by rats' inability to achieve true skeletal maturity and a slight morphology that limits surgical instrumentation. Rabbit models have been described which do not have these limitations, but previous models have relied on adjunctive steroid therapy to achieve osteoporosis and have required animal sacrifice for bone mineral density assessment. Methods: Thirty-six skeletally mature female rabbits underwent bilateral OVX. BMD was measured using dual-energy X-ray absorptiometry on the metaphysis of the proximal tibia and distal femur, at baseline and 17 weeks postoperatively. Results: Mean BMD values were significantly reduced by 21.9% (p < 0.05) in the proximal tibia and 11.9% (p < 0.001) in the distal femur at 17 weeks. Conclusions: This study is the first to demonstrate a significant bone loss within four months of pure OVX in rabbits using animalsparing validation techniques. We believe that this OVX model is safe, reproducible, and can be employed to longitudinally evaluate the effect of anti-osteoporosis therapeutics and surgical interventions.

原文English
頁(從 - 到)12-17
頁數6
期刊Asian Spine Journal
12
發行號1
DOIs
出版狀態Published - 2018 二月 1

All Science Journal Classification (ASJC) codes

  • Surgery
  • Orthopedics and Sports Medicine

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