Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection

Triwit Rattanarojpong, Hao Ching Wang, Chu Fang Lo, Timothy W. Flegel

研究成果: Article同行評審

41 引文 斯高帕斯(Scopus)

摘要

In this proteomic analysis of gills from yellow head virus (YHV)-infected Penaeus vannamei, we identified 13 spots with up-regulated protein expression levels and five spots with down-regulated levels. LC-nanoESI-MS/MS indicated that the up-regulated proteins included enzymes in the glycolytic pathway, the tricarboxylic acid cycle and amino acid metabolism. The other up-regulated proteins were arginine kinase, imaginal disk growth factor (IDGF) and a Ras-like GTP binding protein. By contrast, expression levels were reduced for an SCP-calcium binding protein (SCP), actin-1, a valosin-containing protein, and Rab11. Time-course assays by real time RT-PCR revealed no significant increase in mRNA level of glycolytic enzymes and arginine kinase. However, a significant decrease in SCP mRNA was observed. The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general.

原文English
頁(從 - 到)3809-3814
頁數6
期刊Proteomics
7
發行號20
DOIs
出版狀態Published - 2007 十月 1

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 分子生物學

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