Analysis of interferon gamma protein expression in zebrafish (Danio rerio)

Sohye Yoon, Ayham Alnabulsi, Ting Yu Wang, Po Tsang Lee, Tzong Yueh Chen, Steve Bird, Jun Zou, Christopher J. Secombes

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

IFN-γ is a major effector cytokine, produced to induce type I immune responses. It has been cloned in several fish species including zebrafish, however to date few studies have looked at IFN-γ protein expression and bioactivity in fish. Hence, the current study focused on developing a monoclonal antibody (moAb) against zfIFN-γ. We show that the zfIFN-γ moAb specifically recognises E. coli produced recombinant IFN-γ protein and zfIFN-γ produced in transfected HEK293 cells, by Western blot analysis. Next we analysed the production of the native protein following expression induced by PHA stimulation of leukocytes in vitro or antigen re-stimulation in vivo. We show the IFN-γ protein is produced as a dimer, and that a good correlation exists between transcript expression levels and protein levels.

原文English
頁(從 - 到)79-86
頁數8
期刊Fish and Shellfish Immunology
57
DOIs
出版狀態Published - 2016 十月 1

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

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