Analysis of protein phosphorylation using mass spectrometry

Hsin Yi Wu, Pao-Chi Liao

研究成果: Review article

4 引文 (Scopus)

摘要

Protein phosphorylation has been known to be a pivotal modification regulating many cellular activities and functions. Except for several conventional techniques, mass spectrometry-based strategies are increasingly considered as vital tools that can be utilized to characterize phosphorylated peptides or proteins. In this article, we summarized currently available mass spectrometry-based techniques for the analysis of phosphorylation. Due to the low abundance of phosphopeptides, enrichment steps such as specific antibodies, immobilized metal affinity chromatography, and specific tags are crucial for their use in detection. Since the non-specific binding of the enrichment techniques are constantly of major concerns, phosphatase treatment, neutral loss scan, or precursor ion scan enable the recognition of the phosphopeptide signals. In addition, quantitative methods including isotope labeling and mass tags are also discussed. Phosphoproteome analysis seems to provide elucidation of signaling networks and global decipherment of cell activities, which require powerful analytical methods for complete and routine identification of the phosphorylation event. Despite that numerous approaches have been exploited, comprehensive analysis of protein phosphorylation remains a challenging task. With the progressively more improvements of instruments and methodologies, we can foresee the implementation of a comprehensive approach for the analysis of phosphorylation states of proteins.

原文English
頁(從 - 到)217-227
頁數11
期刊Chang Gung Medical Journal
31
發行號3
出版狀態Published - 2008 五月

指紋

Mass Spectrometry
Phosphorylation
Phosphopeptides
Proteins
Immobilized Antibodies
Isotope Labeling
Affinity Chromatography
Phosphoric Monoester Hydrolases
Metals
Ions
Peptides

All Science Journal Classification (ASJC) codes

  • Medicine(all)

引用此文

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Analysis of protein phosphorylation using mass spectrometry. / Wu, Hsin Yi; Liao, Pao-Chi.

於: Chang Gung Medical Journal, 卷 31, 編號 3, 05.2008, p. 217-227.

研究成果: Review article

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