Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells

Bing Hung Chen; Mei Huei Hung; Jeff Yi Fu Chen; Hsueh Wei Chang; Meng Lung Yu; Lei Wan; Fuu Jen Tsai; Tzu Pin Wang; Tzu Fun Fu; Chien Chih Chiu

doi:10.1016/j.foodchem.2011.11.079

Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells

Bing Hung Chen
, Mei Huei Hung
, Jeff Yi Fu Chen
, Hsueh Wei Chang
, Meng Lung Yu
, Lei Wan
, Fuu Jen Tsai
, Tzu Pin Wang
, Tzu Fun Fu
, Chien Chih Chiu

醫學檢驗生物技術學系

研究成果: Article › 同行評審

33 引文斯高帕斯（Scopus）

摘要

Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca ²⁺ influx upon antigen stimulation was inhibited. Suppression on FcRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases.

原文	English
頁（從 - 到）	968-974
頁數	7
期刊	Food Chemistry
卷	132
發行號	2
DOIs	https://doi.org/10.1016/j.foodchem.2011.11.079
出版狀態	Published - 2012 5月 15

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SDG 3 良好的健康和福祉

All Science Journal Classification (ASJC) codes

分析化學
食品科學

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10.1016/j.foodchem.2011.11.079

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title = "Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells",

abstract = "Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca 2+ influx upon antigen stimulation was inhibited. Suppression on FcRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases.",

author = "Chen, \{Bing Hung\} and Hung, \{Mei Huei\} and Chen, \{Jeff Yi Fu\} and Chang, \{Hsueh Wei\} and Yu, \{Meng Lung\} and Lei Wan and Tsai, \{Fuu Jen\} and Wang, \{Tzu Pin\} and Fu, \{Tzu Fun\} and Chiu, \{Chien Chih\}",

note = "Funding Information: This work was partly supported by Grants from the National Science Council , Taiwan ( NSC95-2320-B-037-045 ), The Department of Health , Taiwan ( DOH100-TD-C-111-002 ) and Kaohsiung Medical University ( KMU-M098007 and KMU-EM-99-1.4 ).",

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AU - Chen, Bing Hung

AU - Hung, Mei Huei

AU - Chen, Jeff Yi Fu

AU - Chang, Hsueh Wei

AU - Yu, Meng Lung

AU - Wan, Lei

AU - Tsai, Fuu Jen

AU - Wang, Tzu Pin

AU - Fu, Tzu Fun

AU - Chiu, Chien Chih

N1 - Funding Information: This work was partly supported by Grants from the National Science Council , Taiwan ( NSC95-2320-B-037-045 ), The Department of Health , Taiwan ( DOH100-TD-C-111-002 ) and Kaohsiung Medical University ( KMU-M098007 and KMU-EM-99-1.4 ).

PY - 2012/5/15

Y1 - 2012/5/15

N2 - Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca 2+ influx upon antigen stimulation was inhibited. Suppression on FcRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases.

AB - Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca 2+ influx upon antigen stimulation was inhibited. Suppression on FcRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases.

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Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells

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