Anti-lewis X antibody promotes Helicobacter pylon adhesion to gastric epithelial cells

Shew Meei Sheu, Bor Shyang Sheu, Hsiao Bai Yang, Huan Yao Lei, Jiunn Jong Wu

研究成果: Article

18 引文 (Scopus)

摘要

Lewis X (Lex) antigen is expressed on the human gastric mucosa and the O-specific chain of lipopolysaccharides of Helicobacter pylori. This antigen can induce autoantibodies, which may be involved in bacterial colonization and thus deserve further investigation. Flow cytometry was used to examine the effects of anti-Le monoclonal antibodies (MAbs) on H. pylori adhesion. A babA2 mutant was also constructed to evaluate the effect of an anti-Lex MAb on adhesion. The bacterial agglutination and in situ adhesion assays were used to confirm the anti-Lex MAb effect on H. pylori adhesion. This study revealed that an anti-Lex MAb, but not an anti-Leb MAb or an anti-Ley MAb, could enhance the adhesion of H. pylori strains that expressed high levels of Lex antigen to AGS cells. The enhancement was not found on an H. pylori strain with a low level of Lex antigen. Anti-Lex MAb could increase the adhesion of both the wild-type strain and its isogenic babA2 mutant to AGS cells. When AGS cells were pretreated with anti-Lex MAb, the adhesion of the babA2 mutant also increased. Only anti-Lex MAb could promote bacterial agglutination, and the in situ adhesion assay further confirmed that adding anti-Lex MAb resulted in denser bacterial adhesion on the gastric epithelia collected from clinical patients. These results suggest anti-Lex MAb could specifically enhance the adhesion abilities of H. pylori strains through a mechanism by which anti-Lex MAb promotes bacterial aggregation and mediates bivalent interaction (antigen-antibody- antigen) between bacteria and host cells.

原文English
頁(從 - 到)2661-2667
頁數7
期刊Infection and Immunity
75
發行號6
DOIs
出版狀態Published - 2007 六月 1

指紋

Helicobacter
Helicobacter pylori
CD15 Antigens
Stomach
Epithelial Cells
Antibodies
Agglutination
Antigens
Bacterial Adhesion
Gastric Mucosa
Autoantibodies
Flow Cytometry
Epithelium
Monoclonal Antibodies
Bacteria

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

引用此文

Sheu, Shew Meei ; Sheu, Bor Shyang ; Yang, Hsiao Bai ; Lei, Huan Yao ; Wu, Jiunn Jong. / Anti-lewis X antibody promotes Helicobacter pylon adhesion to gastric epithelial cells. 於: Infection and Immunity. 2007 ; 卷 75, 編號 6. 頁 2661-2667.
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abstract = "Lewis X (Lex) antigen is expressed on the human gastric mucosa and the O-specific chain of lipopolysaccharides of Helicobacter pylori. This antigen can induce autoantibodies, which may be involved in bacterial colonization and thus deserve further investigation. Flow cytometry was used to examine the effects of anti-Le monoclonal antibodies (MAbs) on H. pylori adhesion. A babA2 mutant was also constructed to evaluate the effect of an anti-Lex MAb on adhesion. The bacterial agglutination and in situ adhesion assays were used to confirm the anti-Lex MAb effect on H. pylori adhesion. This study revealed that an anti-Lex MAb, but not an anti-Leb MAb or an anti-Ley MAb, could enhance the adhesion of H. pylori strains that expressed high levels of Lex antigen to AGS cells. The enhancement was not found on an H. pylori strain with a low level of Lex antigen. Anti-Lex MAb could increase the adhesion of both the wild-type strain and its isogenic babA2 mutant to AGS cells. When AGS cells were pretreated with anti-Lex MAb, the adhesion of the babA2 mutant also increased. Only anti-Lex MAb could promote bacterial agglutination, and the in situ adhesion assay further confirmed that adding anti-Lex MAb resulted in denser bacterial adhesion on the gastric epithelia collected from clinical patients. These results suggest anti-Lex MAb could specifically enhance the adhesion abilities of H. pylori strains through a mechanism by which anti-Lex MAb promotes bacterial aggregation and mediates bivalent interaction (antigen-antibody- antigen) between bacteria and host cells.",
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Anti-lewis X antibody promotes Helicobacter pylon adhesion to gastric epithelial cells. / Sheu, Shew Meei; Sheu, Bor Shyang; Yang, Hsiao Bai; Lei, Huan Yao; Wu, Jiunn Jong.

於: Infection and Immunity, 卷 75, 編號 6, 01.06.2007, p. 2661-2667.

研究成果: Article

TY - JOUR

T1 - Anti-lewis X antibody promotes Helicobacter pylon adhesion to gastric epithelial cells

AU - Sheu, Shew Meei

AU - Sheu, Bor Shyang

AU - Yang, Hsiao Bai

AU - Lei, Huan Yao

AU - Wu, Jiunn Jong

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N2 - Lewis X (Lex) antigen is expressed on the human gastric mucosa and the O-specific chain of lipopolysaccharides of Helicobacter pylori. This antigen can induce autoantibodies, which may be involved in bacterial colonization and thus deserve further investigation. Flow cytometry was used to examine the effects of anti-Le monoclonal antibodies (MAbs) on H. pylori adhesion. A babA2 mutant was also constructed to evaluate the effect of an anti-Lex MAb on adhesion. The bacterial agglutination and in situ adhesion assays were used to confirm the anti-Lex MAb effect on H. pylori adhesion. This study revealed that an anti-Lex MAb, but not an anti-Leb MAb or an anti-Ley MAb, could enhance the adhesion of H. pylori strains that expressed high levels of Lex antigen to AGS cells. The enhancement was not found on an H. pylori strain with a low level of Lex antigen. Anti-Lex MAb could increase the adhesion of both the wild-type strain and its isogenic babA2 mutant to AGS cells. When AGS cells were pretreated with anti-Lex MAb, the adhesion of the babA2 mutant also increased. Only anti-Lex MAb could promote bacterial agglutination, and the in situ adhesion assay further confirmed that adding anti-Lex MAb resulted in denser bacterial adhesion on the gastric epithelia collected from clinical patients. These results suggest anti-Lex MAb could specifically enhance the adhesion abilities of H. pylori strains through a mechanism by which anti-Lex MAb promotes bacterial aggregation and mediates bivalent interaction (antigen-antibody- antigen) between bacteria and host cells.

AB - Lewis X (Lex) antigen is expressed on the human gastric mucosa and the O-specific chain of lipopolysaccharides of Helicobacter pylori. This antigen can induce autoantibodies, which may be involved in bacterial colonization and thus deserve further investigation. Flow cytometry was used to examine the effects of anti-Le monoclonal antibodies (MAbs) on H. pylori adhesion. A babA2 mutant was also constructed to evaluate the effect of an anti-Lex MAb on adhesion. The bacterial agglutination and in situ adhesion assays were used to confirm the anti-Lex MAb effect on H. pylori adhesion. This study revealed that an anti-Lex MAb, but not an anti-Leb MAb or an anti-Ley MAb, could enhance the adhesion of H. pylori strains that expressed high levels of Lex antigen to AGS cells. The enhancement was not found on an H. pylori strain with a low level of Lex antigen. Anti-Lex MAb could increase the adhesion of both the wild-type strain and its isogenic babA2 mutant to AGS cells. When AGS cells were pretreated with anti-Lex MAb, the adhesion of the babA2 mutant also increased. Only anti-Lex MAb could promote bacterial agglutination, and the in situ adhesion assay further confirmed that adding anti-Lex MAb resulted in denser bacterial adhesion on the gastric epithelia collected from clinical patients. These results suggest anti-Lex MAb could specifically enhance the adhesion abilities of H. pylori strains through a mechanism by which anti-Lex MAb promotes bacterial aggregation and mediates bivalent interaction (antigen-antibody- antigen) between bacteria and host cells.

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