Antibacterial Activity of Cysteine-Derived Cationic Dipeptides

Yu Cheng Tsai, Chen Chi Tang, Hsu Heng Wu, Yu Shiang Wang, Yu-Fon Chen

研究成果: Article

摘要

Antibiotic resistance is a growing problem, especially in the treating of life-threatening diseases like sepsis. One way to address such an issue is with the use of antimicrobial peptides, which can kill many types of bacteria by disrupting cellular targets (such as membranes) through electrostatic interaction. In this report, cysteine-derived cationic dipeptides lysine–cysteine (KC), arginine–cysteine (RC) and histidine–cysteine (HC) were used to evaluate antibacterial activity against Gram-negative and positive bacteria. The dipeptides exhibited bacterial membrane rupture capabilities under SEM observation after treatment with IC50 conditions, as well as low cytotoxicity and hemolytic activity toward normal cell lines and human red blood cells (RBCs) at IC50. Furthermore, the dipeptides significantly ameliorated Enterohaemorrhagic E. coli (EHEC)-induced lethality in Caenorhabditis elegans in a dose-dependent manner. These cysteine-derived cationic dipeptides may provide a novel alternative therapy in combating bacterial infection.

原文English
期刊International Journal of Peptide Research and Therapeutics
DOIs
出版狀態Accepted/In press - 2019 一月 1

指紋

Dipeptides
Cysteine
Inhibitory Concentration 50
Bacteria
Cells
Enterohemorrhagic Escherichia coli
Membranes
Caenorhabditis elegans
Gram-Positive Bacteria
Antibiotics
Cytotoxicity
Complementary Therapies
Microbial Drug Resistance
Coulomb interactions
Gram-Negative Bacteria
Static Electricity
Bacterial Infections
Escherichia coli
Peptides
Rupture

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Bioengineering
  • Biochemistry
  • Molecular Medicine
  • Drug Discovery

引用此文

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title = "Antibacterial Activity of Cysteine-Derived Cationic Dipeptides",
abstract = "Antibiotic resistance is a growing problem, especially in the treating of life-threatening diseases like sepsis. One way to address such an issue is with the use of antimicrobial peptides, which can kill many types of bacteria by disrupting cellular targets (such as membranes) through electrostatic interaction. In this report, cysteine-derived cationic dipeptides lysine–cysteine (KC), arginine–cysteine (RC) and histidine–cysteine (HC) were used to evaluate antibacterial activity against Gram-negative and positive bacteria. The dipeptides exhibited bacterial membrane rupture capabilities under SEM observation after treatment with IC50 conditions, as well as low cytotoxicity and hemolytic activity toward normal cell lines and human red blood cells (RBCs) at IC50. Furthermore, the dipeptides significantly ameliorated Enterohaemorrhagic E. coli (EHEC)-induced lethality in Caenorhabditis elegans in a dose-dependent manner. These cysteine-derived cationic dipeptides may provide a novel alternative therapy in combating bacterial infection.",
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Antibacterial Activity of Cysteine-Derived Cationic Dipeptides. / Tsai, Yu Cheng; Tang, Chen Chi; Wu, Hsu Heng; Wang, Yu Shiang; Chen, Yu-Fon.

於: International Journal of Peptide Research and Therapeutics, 01.01.2019.

研究成果: Article

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AU - Tsai, Yu Cheng

AU - Tang, Chen Chi

AU - Wu, Hsu Heng

AU - Wang, Yu Shiang

AU - Chen, Yu-Fon

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Antibiotic resistance is a growing problem, especially in the treating of life-threatening diseases like sepsis. One way to address such an issue is with the use of antimicrobial peptides, which can kill many types of bacteria by disrupting cellular targets (such as membranes) through electrostatic interaction. In this report, cysteine-derived cationic dipeptides lysine–cysteine (KC), arginine–cysteine (RC) and histidine–cysteine (HC) were used to evaluate antibacterial activity against Gram-negative and positive bacteria. The dipeptides exhibited bacterial membrane rupture capabilities under SEM observation after treatment with IC50 conditions, as well as low cytotoxicity and hemolytic activity toward normal cell lines and human red blood cells (RBCs) at IC50. Furthermore, the dipeptides significantly ameliorated Enterohaemorrhagic E. coli (EHEC)-induced lethality in Caenorhabditis elegans in a dose-dependent manner. These cysteine-derived cationic dipeptides may provide a novel alternative therapy in combating bacterial infection.

AB - Antibiotic resistance is a growing problem, especially in the treating of life-threatening diseases like sepsis. One way to address such an issue is with the use of antimicrobial peptides, which can kill many types of bacteria by disrupting cellular targets (such as membranes) through electrostatic interaction. In this report, cysteine-derived cationic dipeptides lysine–cysteine (KC), arginine–cysteine (RC) and histidine–cysteine (HC) were used to evaluate antibacterial activity against Gram-negative and positive bacteria. The dipeptides exhibited bacterial membrane rupture capabilities under SEM observation after treatment with IC50 conditions, as well as low cytotoxicity and hemolytic activity toward normal cell lines and human red blood cells (RBCs) at IC50. Furthermore, the dipeptides significantly ameliorated Enterohaemorrhagic E. coli (EHEC)-induced lethality in Caenorhabditis elegans in a dose-dependent manner. These cysteine-derived cationic dipeptides may provide a novel alternative therapy in combating bacterial infection.

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